PDF(Pubmed)
Abstract：
Understanding the response of soil microbial communities to pathogenic Ralstonia solanacearum is crucial for preventing bacterial wilt outbreaks. In this study, we investigated the soil physicochemical and microbial community to assess their impact on the pathogenic R.solanacearum through metagenomics. Our results revealed that certain archaeal taxa were the main contributors influencing the health of plants. Additionally, the presence of the pathogen showed a strong negative correlation with soil phosphorus levels, while soil phosphorus was significantly correlated with bacterial and archaeal communities. We found that the network of microbial interactions in healthy plant rhizosphere soils was more complex compared to diseased soils. The diseased soil network had more linkages, particularly related to the pathogen occurrence. Within the network, the family Comamonadaceae, specifically Ramlibacter_tataouinensis, was enriched in healthy samples and showed a significantly negative correlation with the pathogen. In terms of archaea, Halorubrum, Halorussus_halophilus (family: Halobacteriaceae), and Natronomonas_pharaonis (family: Haloarculaceae) were enriched in healthy plant rhizosphere soils and showed negative correlations with R.solanacearum. These findings suggested that the presence of these archaea may potentially reduce the occurrence of bacterial wilt disease. On the other hand, Halostagnicola_larseniia and Haloterrigena_sp._BND6 (family: Natrialbaceae) had higher relative abundance in diseased plants and exhibited significantly positive correlations with R.solanacearum, indicating their potential contribution to the pathogen\'s occurrence. Moreover, we explored the possibility of functional gene sharing among the correlating bacterial pairs within the Molecular Ecological Network. Our analysis revealed 468 entries of horizontal gene transfer (HGT) events, emphasizing the significance of HGT in shaping the adaptive traits of plant-associated bacteria, particularly in relation to host colonization and pathogenicity. Overall, this work revealed key factors, patterns and response mechanisms underlying the rhizosphere soil microbial populations. The findings offer valuable guidance for effectively controlling soil-borne bacterial diseases and developing sustainable agriculture practices.
摘要：
了解土壤微生物群落对致病性青枯雷尔氏菌的反应对于预防青枯病暴发至关重要。在这项研究中,我们通过宏基因组学研究了土壤理化和微生物群落，以评估它们对致病性龙葵的影响。我们的结果表明，某些古细菌类群是影响植物健康的主要因素。此外,病原体的存在与土壤磷水平呈强烈的负相关，而土壤磷与细菌和古细菌群落显著相关。我们发现，与患病土壤相比，健康植物根际土壤中的微生物相互作用网络更为复杂。患病的土壤网络有更多的联系，特别是与病原体的发生有关。在网络中,科从科，特别是塔塔瓦因氏杆菌，在健康样本中富集，并与病原体呈显著负相关。就古细菌而言，Halorubrum,Halorussus_halophilus（家族：Halobacteriaceae），和自然单胞菌（科：Haloarculaceae）在健康的植物根际土壤中富集，并与茄科R.solanacearuma呈负相关。这些发现表明，这些古细菌的存在可能会减少细菌性枯萎病的发生。另一方面,Halostagnicola_larseniia和Haloterrigena_sp。_BND6（家族：Natrialbaceae）在患病植物中具有较高的相对丰度，并且与青枯树呈显着正相关，表明它们对病原体发生的潜在贡献。此外，我们探索了分子生态网络中相关细菌对之间功能基因共享的可能性。我们的分析揭示了468个水平基因转移（HGT）事件，强调HGT在塑造植物相关细菌的适应性性状方面的重要性，特别是与宿主定植和致病性有关。总的来说,这项工作揭示了关键因素，根际土壤微生物种群的模式和响应机制。研究结果为有效控制土壤传播的细菌性疾病和发展可持续农业实践提供了宝贵的指导。
