PDF(Pubmed)
Abstract：
The aim of the study was to make a vascular patch based on regenerated silk fibroin (SF) and study its physical and mechanical characteristics, biocompatibility and matrix properties in comparison with polyhydroxybutyrate/valerate/polycaprolactone with incorporated vascular endothelial growth factor (PHBV/PCL/VEGF) and commercial bovine xenopericardium (XP) flap in experiments in vitro.
UNASSIGNED: Tissue-engineered matrices were produced by electrospinning. The surface structure, physical and mechanical characteristics, hemocompatibility (erythrocyte hemolysis, aggregation, adhesion and activation of platelets after contact with the material) and matrix properties of vascular patches (adhesion, viability, metabolic activity of EA.hy926 cells on the material) were studied.
UNASSIGNED: The surface of SF-based matrices and PHBV/PCL/VEGF-based tissue engineered patches had a porous and fibrous structure compared to a denser and more uniform XP flap. The physical and mechanical characteristics of SF matrices were close to those of native vessels. Along with this, tissue-engineered patches demonstrated high hemocompatible properties, which do not differ from those for commercial XP flap. Adhesion, viability, and metabolic activity of EA.hy926 endothelial cells also corresponded to the previously developed PHBV/PCL/VEGF matrix and XP flap, which indicates the nontoxicity and biocompatibility of SF matrices.
UNASSIGNED: Matrices produced from regenerated SF demonstrated satisfactory results, comparable to those for PHBV/PCL/VEGF and commercial XP flap, and in the case of platelet adhesion and activation, they outperformed these patches. In total, SF can be defined as material having sufficient biological compatibility, which makes it possible to consider a tissue-engineered matrix made from it as promising for implantation into the vascular wall.
UNASSIGNED: Tissue-engineered matrices were produced by electrospinning. The surface structure, physical and mechanical characteristics, hemocompatibility (erythrocyte hemolysis, aggregation, adhesion and activation of platelets after contact with the material) and matrix properties of vascular patches (adhesion, viability, metabolic activity of EA.hy926 cells on the material) were studied.
UNASSIGNED: The surface of SF-based matrices and PHBV/PCL/VEGF-based tissue engineered patches had a porous and fibrous structure compared to a denser and more uniform XP flap. The physical and mechanical characteristics of SF matrices were close to those of native vessels. Along with this, tissue-engineered patches demonstrated high hemocompatible properties, which do not differ from those for commercial XP flap. Adhesion, viability, and metabolic activity of EA.hy926 endothelial cells also corresponded to the previously developed PHBV/PCL/VEGF matrix and XP flap, which indicates the nontoxicity and biocompatibility of SF matrices.
UNASSIGNED: Matrices produced from regenerated SF demonstrated satisfactory results, comparable to those for PHBV/PCL/VEGF and commercial XP flap, and in the case of platelet adhesion and activation, they outperformed these patches. In total, SF can be defined as material having sufficient biological compatibility, which makes it possible to consider a tissue-engineered matrix made from it as promising for implantation into the vascular wall.
摘要：
本研究旨在制备基于再生丝素蛋白(SF)的血管补片,并研究其物理力学特性,在体外实验中，与掺入血管内皮生长因子（PHBV/PCL/VEGF）的聚羟基丁酸酯/戊酸酯/聚己内酯和商业牛异种心包（XP）皮瓣相比，生物相容性和基质特性。
■通过静电纺丝产生组织工程化基质。表面结构，物理和机械特性，血液相容性（红细胞溶血，聚合，与材料接触后血小板的粘附和活化）和血管斑块的基质特性（粘附，生存能力，EA的代谢活动。材料上的hy926细胞）进行了研究。
■与更致密，更均匀的XP瓣相比，基于SF的基质和基于PHBV/PCL/VEGF的组织工程贴片的表面具有多孔和纤维结构。SF基质的物理和机械特性接近天然血管。伴随着这个,组织工程贴片表现出很高的血液相容性，这与商业XP皮瓣没有什么不同。附着力,生存能力，和EA的代谢活动。hy926内皮细胞也对应于先前开发的PHBV/PCL/VEGF基质和XP皮瓣，表明SF基质的无毒性和生物相容性。
■由再生SF产生的矩阵显示出令人满意的结果，与PHBV/PCL/VEGF和商用XP皮瓣相当，在血小板粘附和活化的情况下，他们的表现优于这些补丁。总的来说,SF可以定义为具有足够生物相容性的材料，这使得有可能认为由它制成的组织工程基质有望植入血管壁。
■通过静电纺丝产生组织工程化基质。表面结构，物理和机械特性，血液相容性（红细胞溶血，聚合，与材料接触后血小板的粘附和活化）和血管斑块的基质特性（粘附，生存能力，EA的代谢活动。材料上的hy926细胞）进行了研究。
■与更致密，更均匀的XP瓣相比，基于SF的基质和基于PHBV/PCL/VEGF的组织工程贴片的表面具有多孔和纤维结构。SF基质的物理和机械特性接近天然血管。伴随着这个,组织工程贴片表现出很高的血液相容性，这与商业XP皮瓣没有什么不同。附着力,生存能力，和EA的代谢活动。hy926内皮细胞也对应于先前开发的PHBV/PCL/VEGF基质和XP皮瓣，表明SF基质的无毒性和生物相容性。
■由再生SF产生的矩阵显示出令人满意的结果，与PHBV/PCL/VEGF和商用XP皮瓣相当，在血小板粘附和活化的情况下，他们的表现优于这些补丁。总的来说,SF可以定义为具有足够生物相容性的材料，这使得有可能认为由它制成的组织工程基质有望植入血管壁。
