PDF(Pubmed)
Abstract：
UNASSIGNED: There are limited longitudinal data on the systemic and mucosal antibody responses to SARS-CoV-2 from Latin America, a region severely affected by COVID-19, and where vaccine strategies have been implemented during the evolving pandemic.
UNASSIGNED: To evaluate determinants of seroprevalence and changes in levels of anti-SARS-CoV-2 antibodies longitudinally in adults with different levels of exposure to SARS-CoV-2 (defined a priori as low, medium, and high based on presumed occupational risk), in two Andean cities in Ecuador.
UNASSIGNED: Longitudinal cohort study of 1,000 adults aged 18 years and older with questionnaire data and sample collection done at 0, 3, 6, and 12 months during the period 2020-2023. Observations collected included WHO-ISARIC questionnaire and peripheral blood and saliva samples for measurement of IgG and IgA antibodies, respectively. Planned analyses are tailored to the longitudinal nature of the outcomes defined by participants\' antibody levels and aim at estimating their average trends with time since infection in each of the occupational groups, adjusted for demographics and calendar-time levels of SARS-CoV-2 infection in the general population. The latter reflect the impact of the national control measures such as vaccinations and movement restrictions.
UNASSIGNED: Understanding the duration and the dynamics of waning immunity to SARS-CoV-2, in the context of exposures to emerging virus variants and immunization, will inform the implementation of targeted public health strategies in the Latin American region.
UNASSIGNED: This study will observe the bioethical principles of the Declaration of Helsinki. Informed written consent will be obtained. Samples from participants will be stored for up to three years after which they will be destroyed. The study protocol was approved by the Ecuadorian Ministry of Public Health Ethics Committee for COVID-19 Research. Antibody results will be provided to participants and participating institutions and to the national health authorities.
UNASSIGNED: To evaluate determinants of seroprevalence and changes in levels of anti-SARS-CoV-2 antibodies longitudinally in adults with different levels of exposure to SARS-CoV-2 (defined a priori as low, medium, and high based on presumed occupational risk), in two Andean cities in Ecuador.
UNASSIGNED: Longitudinal cohort study of 1,000 adults aged 18 years and older with questionnaire data and sample collection done at 0, 3, 6, and 12 months during the period 2020-2023. Observations collected included WHO-ISARIC questionnaire and peripheral blood and saliva samples for measurement of IgG and IgA antibodies, respectively. Planned analyses are tailored to the longitudinal nature of the outcomes defined by participants\' antibody levels and aim at estimating their average trends with time since infection in each of the occupational groups, adjusted for demographics and calendar-time levels of SARS-CoV-2 infection in the general population. The latter reflect the impact of the national control measures such as vaccinations and movement restrictions.
UNASSIGNED: Understanding the duration and the dynamics of waning immunity to SARS-CoV-2, in the context of exposures to emerging virus variants and immunization, will inform the implementation of targeted public health strategies in the Latin American region.
UNASSIGNED: This study will observe the bioethical principles of the Declaration of Helsinki. Informed written consent will be obtained. Samples from participants will be stored for up to three years after which they will be destroyed. The study protocol was approved by the Ecuadorian Ministry of Public Health Ethics Committee for COVID-19 Research. Antibody results will be provided to participants and participating institutions and to the national health authorities.
摘要：
■拉丁美洲对SARS-CoV-2的全身和粘膜抗体反应的纵向数据有限，受COVID-19严重影响的地区，在不断发展的大流行期间实施了疫苗策略。
■为了纵向评估暴露于不同SARS-CoV-2水平的成年人的血清阳性率的决定因素和抗SARS-CoV-2抗体水平的变化（先验定义为低，中等,并且基于假定的职业风险较高)，在厄瓜多尔的两个安第斯城市。
■在2020-2023年期间，对1,000名18岁及以上的成年人进行了纵向队列研究，并在0、3、6和12个月进行了问卷调查数据和样本收集。收集的观察包括WHO-ISARIC问卷和外周血和唾液样本，用于测量IgG和IgA抗体，分别。计划分析针对参与者抗体水平定义的结果的纵向性质，旨在估计每个职业群体自感染以来随时间的平均趋势。调整了一般人群中SARS-CoV-2感染的人口统计学和日历时间水平。后者反映了国家控制措施的影响，例如疫苗接种和行动限制。
■在暴露于新出现的病毒变体和免疫的背景下，了解SARS-CoV-2免疫力下降的持续时间和动态，将为拉丁美洲地区实施有针对性的公共卫生战略提供信息。
■本研究将遵循《赫尔辛基宣言》的生物伦理原则。将获得知情的书面同意。参与者的样本将被保存长达三年，之后将被销毁。该研究方案获得厄瓜多尔公共卫生伦理委员会COVID-19研究批准。抗体结果将提供给参与者和参与机构以及国家卫生当局。
■为了纵向评估暴露于不同SARS-CoV-2水平的成年人的血清阳性率的决定因素和抗SARS-CoV-2抗体水平的变化（先验定义为低，中等,并且基于假定的职业风险较高)，在厄瓜多尔的两个安第斯城市。
■在2020-2023年期间，对1,000名18岁及以上的成年人进行了纵向队列研究，并在0、3、6和12个月进行了问卷调查数据和样本收集。收集的观察包括WHO-ISARIC问卷和外周血和唾液样本，用于测量IgG和IgA抗体，分别。计划分析针对参与者抗体水平定义的结果的纵向性质，旨在估计每个职业群体自感染以来随时间的平均趋势。调整了一般人群中SARS-CoV-2感染的人口统计学和日历时间水平。后者反映了国家控制措施的影响，例如疫苗接种和行动限制。
■在暴露于新出现的病毒变体和免疫的背景下，了解SARS-CoV-2免疫力下降的持续时间和动态，将为拉丁美洲地区实施有针对性的公共卫生战略提供信息。
■本研究将遵循《赫尔辛基宣言》的生物伦理原则。将获得知情的书面同意。参与者的样本将被保存长达三年，之后将被销毁。该研究方案获得厄瓜多尔公共卫生伦理委员会COVID-19研究批准。抗体结果将提供给参与者和参与机构以及国家卫生当局。
