Abstract：
Development of osteochondral tissue engineering approaches using scaffolds seeded with stem cells in association with mechanical stimulations has been recently considered as a promising technique for the repair of this tissue. In this study, an integrated and biomimetic trilayered silk fibroin (SF) scaffold containing SF nanofibers in each layer was fabricated. The osteogenesis and chondrogenesis of stem cells seeded on the fabricated scaffolds were investigated under a perfusion flow. 3-Dimethylthiazol-2,5-diphenyltetrazolium bromide assay showed that the perfusion flow significantly enhanced cell viability and proliferation. Analysis of gene expression by stem cells revealed that perfusion flow had significantly upregulated the expression of osteogenic and chondrogenic genes in the bone and cartilage layers and downregulated the hypertrophic gene expression in the intermediate layer of the scaffold. In conclusion, applying flow perfusion on the prepared integrated trilayered SF-based scaffold can support osteogenic and chondrogenic differentiation for repairing osteochondral defects.
摘要：
最近，使用植入有干细胞的支架与机械刺激相结合的骨软骨组织工程方法的开发被认为是修复该组织的有前途的技术。在这项研究中,制备在每一层中含有SF纳米纤维的一体化和仿生三层丝素蛋白(SF)支架。在灌注流下研究了接种在制成的支架上的干细胞的成骨和软骨形成。3-二甲基噻唑-2,5-二苯基四唑溴化物测定显示灌注流显着增强细胞活力和增殖。干细胞的基因表达分析表明，灌注流显着上调了骨和软骨层中成骨和软骨形成基因的表达，并下调了支架中间层中的肥大基因表达。总之,在制备的基于SF的三层集成支架上应用流动灌注可以支持成骨和软骨分化，以修复骨软骨缺损。
