Abstract：
Mycotoxins can be found in food and feed storage as well as in several kinds of foodstuff and are capable of harming mammals and some of them even in small doses. This study investigated on the undifferentiated neuronal cell line SH-SY5Y the effects of two mycotoxins: patulin (PAT) and citrinin (CTN), which are predominantly produced by fungi species Penicillium and Aspergillus. Here, the individual and combined cytotoxicity of PAT and CTN was investigated using the cytotoxic assay MTT. Our findings indicate that after 24 h of treatment, the IC50 value for PAT is 2.01 μM, which decreases at 1.5 μM after 48 h. In contrast, CTN did not attain an IC50 value at the tested concentration. Therefore, we found PAT to be the more toxic compared to CTN. However, the combined treatment suggests an additive toxic effect. With 2,7-dichlorodihydrofluorescin diacetate (DCFH-DA) DCFH-DA assay, ROS generation was demonstrated after CTN treatment, but PAT showed only small changes. The mixture presented a very constant behavior over time. Finally, the median-effect/combination index (CI-) isobologram equation demonstrated an additive effect after 24 h, but an antagonistic effect after 48 h for the interaction of the two mycotoxins.
摘要：
霉菌毒素可以在食品和饲料储存以及几种食品中发现，并且即使在小剂量下也能够伤害哺乳动物和其中一些。这项研究研究了未分化的神经元细胞系SH-SY5Y两种真菌毒素的作用：棒曲霉素（PAT）和citrinin（CTN），主要由真菌青霉和曲霉产生。这里,使用细胞毒性试验MTT研究PAT和CTN的个体和联合细胞毒性。我们的研究结果表明，治疗24小时后，PAT的IC50值为2.01μM，在48小时后下降1.5μM。相比之下，CTN在测试浓度下没有达到IC50值。因此,我们发现与CTN相比，PAT的毒性更大。然而,联合治疗表明有加性毒性作用。用2,7-二氯二氢荧光素二乙酸酯（DCFH-DA）测定DCFH-DA，CTN治疗后证实了ROS的产生，但是PAT只显示了很小的变化。混合物随时间呈现非常恒定的行为。最后,中值效应/组合指数(CI-)等效图方程在24小时后显示出加性效应，但在48小时后对两种霉菌毒素的相互作用具有拮抗作用。
