Twenty periodontitis DS participants (7 females; 25.5 ± 5.6 years of age; 3 with generalized periodontitis) partook in a study involving non-surgical mechanical periodontal therapy, twice daily chlorhexidine gel toothbrushing, chlorhexidine mouthwash, and monthly recalls. The subgingival microbiota profile was followed at baseline, 6-, and 12-months post-operation.
Desulfobulbus, Saccharibacteria (TM7), Tannerella, and Porphyromonas were the major subgingival genera in this DS cohort. Favorable chlorhexidine adjunct non-surgical treatment outcomes were observed, with the relative abundance of Desulfobulbus sp. HMT 041, Saccharibacteria (TM7) [G-1] bacterium HMT 346 or 349, and Tannerella forsythia significantly reduced at the end of the study, but no significant reduction of Porphyromonas gingivalis or Aggregatibacter actinomycetemcomitans could be observed. Relative abundance of Desulfobulbus sp. HMT 041 and T. forsythia were also found to be significantly associated with plaque, bleeding on probing, and probing pocket depth (PPD, in mm) at a site level, while the relative abundance of Halomonas pacifica was negatively associated with PPD.
Successful chlorhexidine adjunct non-surgical treatment with hygiene care was accompanied by a subgingival microbial shift involving certain periodontopathogenic species, except P. gingivalis and A. actinomycetemcomitans. Further investigations are required to clarify the mechanism underpinning the unchanged relative abundance of the above two pathogens despite favorable clinical responses.
DS adults face challenges achieving optimal home care or hygiene for periodontal healing and disease prevention. Chemical adjunct mechanical periodontal therapy plus regular recalls appeared promising clinically and microbiologically, with subgingival periodontopathogenic species reduction. The persistence of A. actinomycetemcomitans and P. gingivalis in subgingival niches post-treatment warrants further investigation.
方法:20名牙周炎DS参与者(7名女性;25.5±5.6岁;3名全身性牙周炎)参与了一项涉及非手术机械牙周治疗的研究,每天两次洗必泰凝胶刷牙,洗必泰漱口水,每月召回。在基线时跟踪牙龈下微生物群概况,6-,术后12个月。
结果:Desulfobulbus,酵母菌(TM7),制革菌,和卟啉单胞菌是该DS队列中的主要龈下属。观察到良好的氯己定辅助非手术治疗结果,具有Desulfobulbussp。的相对丰度。HMT041、酵母菌(TM7)[G-1]菌HMT346或349、连翘单孢菌在研究结束时显著减少,但是没有观察到牙龈卟啉单胞菌或Aggregatit放线菌的显着减少。脱硫球的相对丰度。还发现HMT041和连翘T.连翘与斑块显着相关,探查时出血,和探测袋深度(PPD,单位为mm)在现场水平,而太平洋Halomonas的相对丰度与PPD呈负相关。
结论:成功的氯己定辅助非手术治疗与卫生护理伴随着涉及某些牙周病菌的龈下微生物转移,除了牙龈卟啉单胞菌和放线菌。尽管临床反应良好,但仍需要进一步研究以阐明支持上述两种病原体相对丰度不变的机制。
结论:DS成人在实现牙周愈合和疾病预防的最佳家庭护理或卫生方面面临挑战。化学辅助机械牙周治疗加上定期召回在临床和微生物学上似乎很有希望,随着牙龈下牙周病原种类的减少。放线菌和牙龈卟啉单胞菌在治疗后的龈下壁ni中的持久性值得进一步研究。