Abstract：
Artificial insemination is a widely adopted method in livestock production for various reasons such as health security and genetic improvement. Although sperm motility is of paramount importance in this technique as it directly influences the sperm\'s ability to fertilize the oocyte. In previous research on human sperm, we observed that in vitro supplementation with Origanum Vulgare essential oil significantly improved sperm motility and antioxidant activities, all without negatively affecting the integrity of their DNA. Based on these promising results, we considered it crucial to explore the potential effects of supplementation with this essential oil on sperm of other species. In this study, we studied the effects of oregano essential oil supplementation on sperm motility of (bulls = 15) (dogs = 15) and (rabbits = 9) and the changes that in vitro incubation with this oil could induce on sub-motile sperm populations of different species. The results of the study showed that in vitro oregano essential oil supplementation had a significant impact on sperm motility in the three species studied. This improvement in sperm motility was accompanied by an increase in the proportion of subpopulations with high velocity and progressivity: an increase of (2.16%, 10% and 4.84%) for subpopulation 1, (6.50%, 5.5% and 3.17%) for subpopulation 4 in bulls, dogs and rabbits respectively. While the subpopulations representing low motile and non-progressive sperm have decreased. These results suggest that the use of oregano essential oil can be a beneficial approach to improve sperm motility in different species, which can have important implications for the success of artificial insemination.
摘要：
人工授精是畜牧业生产中广泛采用的一种方法，其原因有多种，如健康安全和遗传改良。尽管精子活力在这项技术中至关重要，因为它直接影响精子使卵母细胞受精的能力。在以往关于人类精子的研究中,我们观察到体外补充牛至精油显着提高精子活力和抗氧化活性，所有这些都不会对其DNA的完整性产生负面影响。基于这些有希望的结果，我们认为探索补充这种精油对其他物种精子的潜在影响至关重要。在这项研究中,我们研究了补充牛至精油对（公牛=15）（狗=15）和（兔子=9）精子运动的影响，以及用这种油体外孵育对不同物种亚运动精子种群的影响。研究结果表明，体外补充牛至精油对所研究的三个物种的精子活力有显著影响。精子运动性的这种改善伴随着具有高速度和进展性的亚群比例的增加：增加了（2.16％，10%和4.84%)的亚群1，(6.50%，5.5%和3.17%)为公牛第4亚群，分别是狗和兔子。而代表低运动性和非进行性精子的亚群减少了。这些结果表明，使用牛至精油可以是改善不同物种精子活力的有益方法，这对人工授精的成功具有重要意义。
