Abstract：
Gynura procumbens (Lour.) Merr., utilized in traditional Chinese medicine, is known for its liver-protective, liver-soothing, and depression-alleviating properties. This research examines the antidepressant and anti-hyperprolactinemia potentials of an ethanol extract from G. procumbens stems (EEGS) and specific metabolites. To model depression and hyperprolactinemia, chronic unpredictable mild stress (CUMS) was induced in mice and risperidone was administered to rats, respectively. Treatments involved administering low (5 mg/kg), medium (25 mg/kg), and high (125 mg/kg) doses of EEGS and certain metabolites to both models. Behavioral assessments were conducted in the CUMS-induced mice, while the CA3 neuronal damage in mice and histopathological alterations in rat mammary glands were evaluated using Nissl and Hematoxylin & Eosin staining techniques, respectively. EEGS decreased immobility times in the forced swimming and tail suspension tests in mice, enhancing their exploration of the central zone. It elevated the serum levels of 5-hydroxytryptamine, norepinephrine, estradiol, luteinizing hormone (LH), and testosterone in mice. Moreover, EEGS restored the neuronal cell arrangement in the CA3 area, reduced interleukin-1beta mRNA production, and increased the expression of interleukin-10 and beta-catenin mRNA. In the context of risperidone-induced hyperprolactinemia, EEGS lowered blood prolactin levels, reduced the dimensions of rat nipples, and enhanced LH, progesterone, and dopamine levels, alongside mitigating mammary hyperplasia. Among the EEGS selected metabolites, the combined effect of chlorogenic acid and trans-p-coumaric acid was found to be more effective than the action of each compound in isolation. Collectively, the findings indicate that EEGS and its selected metabolites offer promising antidepressant benefits while counteracting hyperprolactinemia.
摘要：
金nuraprocumbens(Lour.）合并。,用于中药，以保护肝脏而闻名，肝脏舒缓，和缓解抑郁的特性。这项研究检查了G.procumbens茎（EEGS）和特定代谢物的乙醇提取物的抗抑郁和抗高催乳素血症潜力。建立抑郁和高泌乳素血症模型,在小鼠中诱导慢性不可预测的轻度应激（CUMS），并对大鼠施用利培酮，分别。治疗涉及施用低（5mg/kg），培养基（25mg/kg），和高剂量（125mg/kg）的EEGS和某些代谢物对两种模型。在CUMS诱导的小鼠中进行行为评估，而小鼠的CA3神经元损伤和大鼠乳腺的组织病理学改变使用Nissl和苏木精和伊红染色技术进行评估，分别。EEGS在小鼠的强迫游泳和尾部悬吊试验中减少了不动时间，加强对中部地区的探索。它提高了5-羟色胺的血清水平，去甲肾上腺素,雌二醇,黄体生成素(LH),和老鼠的睾丸激素。此外，EEGS恢复了CA3区的神经元细胞排列，白细胞介素-1βmRNA产生减少，并增加白细胞介素-10和β-连环蛋白mRNA的表达。在利培酮诱导的高催乳素血症的背景下，EEGS降低了血液催乳素水平，缩小了老鼠乳头的尺寸，增强LH，黄体酮,和多巴胺水平，同时减轻乳腺增生。在EEGS选择的代谢物中，发现绿原酸和反式对香豆酸的联合作用比分离每种化合物的作用更有效。总的来说，研究结果表明,EEGS及其选定的代谢产物在对抗高催乳素血症的同时,提供了有希望的抗抑郁益处.
