PDF(Pubmed)
Abstract：
Glanders, caused by Burkholderia mallei, is a zoonotic disease of equids. Serologic testing for glanders is required by disease-free countries before international movement of equids. The World Organisation for Animal Health Terrestrial Manual recommends the complement fixation test (CFT) for clearance of individual animals for movement, but the CFT is prone to false-positive results. A colorimetric western blot (WB) assay was developed and validated to resolve false-positive CFT results; however, that assay is relatively time-consuming, and the interpretation is subjective. We present here a procedurally similar chemiluminescent WB assay that performs comparably to the validated colorimetric WB assay and offers noticeable benefits of decreased time-to-result and greater ease of interpretation.
摘要：
格兰德斯,由BurkholderiaMallei引起的,是一种人畜共患病。无病国家在国际运医之前需要对腺体进行血清学检测。世界动物卫生组织陆地手册建议使用补体固定试验（CFT）清除单个动物的运动，但CFT容易出现假阳性结果。开发并验证了比色蛋白质印迹（WB）测定法，以解决假阳性的CFT结果；但是，这种检测相对耗时，解释是主观的。我们在这里提出了一种程序上相似的化学发光WB测定，其性能与经过验证的比色WB测定相当，并且提供了减少的结果时间和更容易解释的显着好处。
