PDF(Pubmed)
Abstract：
Numerous studies have investigated the effects of stannous ions on specific microbes and their efficacy in reducing dental plaque. Nonetheless, our understanding of their impact on the oral microbiome is still a subject of ongoing exploration. Therefore, this study sought to evaluate the effects of a stannous-containing sodium fluoride dentifrice in comparison to a zinc-containing sodium fluoride dentifrice and a control group on intact, healthy oral biofilms. Utilizing the novel 2bRAD-M approach for species-resolved metagenomics, and FISH/CLSM with probes targeting periodontal and caries associated species alongside Sn2+ and Zn2+ ions, we collected and analyzed in situ biofilms from 15 generally healthy individuals with measurable dental plaque and treated the biofilms with dentifrices to elucidate variations in microbial distribution. Although significant shifts in the microbiome upon treatment were not observed, the use of a stannous-containing sodium fluoride dentifrice primarily led to an increase in health-associated commensal species and decrease in pathogenic species. Notably, FISH/CLSM analysis highlighted a marked reduction in representative species associated with periodontitis and caries following treatment with the use of a stannous-containing sodium fluoride dentifrice, as opposed to a zinc-containing sodium fluoride dentifrice and the control group. Additionally, Sn2+ specific intracellular imaging reflected the colocalization of Sn2+ ions with P. gingivalis but not with other species. In contrast, Zn2+ ions exhibited non-specific binding, thus suggesting that Sn2+ could exhibit selective binding toward pathogenic species. Altogether, our results demonstrate that stannous ions could help to maintain a healthy oral microbiome by preferentially targeting certain pathogenic bacteria to reverse dysbiosis and underscores the importance of the continual usage of such products as a preventive measure for oral diseases and the maintenance of health.
摘要：
许多研究已经研究了亚锡离子对特定微生物的影响及其在减少牙菌斑中的功效。尽管如此，我们对它们对口腔微生物组的影响的理解仍是一个正在进行探索的课题.因此,这项研究试图评估含亚锡的氟化钠洁牙剂与含锌的氟化钠洁牙剂和对照组相比，健康的口腔生物膜。利用新的2bRAD-M方法进行物种分辨宏基因组学，和FISH/CLSM，与Sn2和Zn2离子一起靶向牙周和龋齿相关物种的探针，我们收集并分析了15名具有可测量牙菌斑的一般健康个体的原位生物膜,并用牙膏处理生物膜以阐明微生物分布的变化.尽管未观察到治疗后微生物组的显着变化，使用含亚锡的氟化钠洁牙剂主要导致与健康相关的共生物种增加和致病物种减少。值得注意的是,FISH/CLSM分析强调了使用含亚锡的氟化钠牙粉治疗后，与牙周炎和龋齿相关的代表性物种显着减少，与含锌氟化钠洁齿剂和对照组相反。此外,Sn2特异性细胞内成像反映了Sn2离子与牙龈卟啉单胞菌的共定位，但不反映与其他物种的共定位。相比之下,Zn2+离子表现出非特异性结合，因此表明Sn2可以对致病物种表现出选择性结合。总之,我们的研究结果表明,亚锡离子可以通过优先靶向某些致病菌来逆转菌群失调,从而有助于维持健康的口腔微生物组,并强调了持续使用此类产品作为口腔疾病预防措施和维护健康的重要性.
