UNASSIGNED: In the present study, these domains as well as the combination of the constitutively expressed FnIII domains 7 and 8 (78) were expressed in Chinese hamster ovary cells as pseudo-antibodies fused to the Fc-fragment of a human immunoglobulin G antibody. The fusion proteins were presented to primary mouse neural stem/progenitor cells (NSPCs) grown as neurospheres, either as coated culture substrates or as soluble additives in vitro. The influence of the domains on the differentiation, proliferation and migration of NSPCs was analyzed.
UNASSIGNED: We observed that the domain combination A124BCD promoted the differentiation of neurons and oligodendrocytes, whereas the domain A1D supported astrocyte differentiation. The constitutively expressed domain 78 had a proliferation and migration stimulating impact. Moreover, most effects were seen only in one of the presentation modes but not in both, suggesting different effects of the Tnc domains in two- and three-dimensional cultures.
UNASSIGNED: This knowledge about the different effect of the Tnc domains might be used to create artificial three-dimensional environments for cell transplantation. Hydrogels spiked with Tnc-domains might represent a promising tool in regenerative medicine.
■在本研究中,这些结构域以及组成型表达的FnIII结构域7和8(78)的组合在中国仓鼠卵巢细胞中表达为与人免疫球蛋白G抗体的Fc片段融合的假抗体。将融合蛋白呈递至生长为神经球的原代小鼠神经干/祖细胞(NSPCs),作为包被的培养基质或作为体外可溶性添加剂。域对分化的影响,分析了NSPCs的增殖和迁移。
■我们观察到结构域组合A124BCD促进了神经元和少突胶质细胞的分化,而结构域A1D支持星形胶质细胞分化。组成型表达的结构域78具有增殖和迁移刺激作用。此外,大多数效果只在一种演示模式中看到,而不是在两种模式中看到,表明Tnc域在二维和三维培养中的不同作用。
■关于Tnc域的不同作用的这种知识可能用于创建用于细胞移植的人造三维环境。掺有Tnc结构域的水凝胶可能代表再生医学中的有前途的工具。