PDF(Pubmed)
Abstract：
BACKGROUND: Arboviral diseases are a group of infectious diseases caused by viruses transmitted by arthropods, mainly mosquitoes. These diseases, such as those caused by the dengue (DENV), Zika (ZIKV), chikungunya (CHIKV), and yellow fever (YFV) viruses, have a significant impact worldwide. In this context, entomological surveillance plays a crucial role in the control and prevention of arboviruses by providing essential information on the presence, distribution, and activity of vector mosquitoes. Based on entomological surveillance, transovarian transmission provides information regarding the maintenance and dissemination of arboviruses. The objective of this study was to detect these arboviruses in Goiânia, Goiás, and analyze the occurrence of transovarian transmission.
METHODS: Aedes aegypti eggs were collected from different regions of Goiânia and cultivated under controlled laboratory conditions until the emergence of adult mosquitoes. Adult females were grouped into pools containing their heads and thoraxes. These pools were subsequently evaluated using reverse-transcription quantitative polymerase chain reaction (RT-qPCR) assay.
RESULTS: A total of 157 pools (N=1570) were analyzed, with two pools testing positive for CHIKV and one pool testing positive for ZIKV, indicating that the offspring resulting from transovarian transmission are potentially infectious.
CONCLUSIONS: In summary, the demonstration of the vertical transmission mechanisms of CHIKV and ZIKV in A. aegypti serves as an alert to health authorities, as these diseases are still underreported, and their primary urban vector has likely acquired this capacity, contributing to the dissemination of these infections.
METHODS: Aedes aegypti eggs were collected from different regions of Goiânia and cultivated under controlled laboratory conditions until the emergence of adult mosquitoes. Adult females were grouped into pools containing their heads and thoraxes. These pools were subsequently evaluated using reverse-transcription quantitative polymerase chain reaction (RT-qPCR) assay.
RESULTS: A total of 157 pools (N=1570) were analyzed, with two pools testing positive for CHIKV and one pool testing positive for ZIKV, indicating that the offspring resulting from transovarian transmission are potentially infectious.
CONCLUSIONS: In summary, the demonstration of the vertical transmission mechanisms of CHIKV and ZIKV in A. aegypti serves as an alert to health authorities, as these diseases are still underreported, and their primary urban vector has likely acquired this capacity, contributing to the dissemination of these infections.
摘要：
背景：虫媒病毒病是由节肢动物传播的病毒引起的一组传染病，主要是蚊子。这些疾病,例如由登革热（DENV）引起的，Zika(ZIKV),基孔肯雅(CHIKV),黄热病（YFV）病毒，在世界范围内产生重大影响。在这种情况下，昆虫监测在控制和预防虫媒病毒方面发挥着至关重要的作用，分布，和媒介蚊子的活动。基于昆虫学监测,经卵巢传播提供了有关虫媒病毒维持和传播的信息。这项研究的目的是在戈亚尼亚中检测这些虫媒病毒，戈亚斯,并分析经卵巢传播的发生情况。
方法：从戈伊尼亚的不同地区收集埃及伊蚊卵，并在受控的实验室条件下培养，直到成年蚊子出现。成年雌性被分组到包含头部和胸部的池中。随后使用逆转录定量聚合酶链反应(RT-qPCR)测定来评估这些池。
结果：共分析了157个池（N=1570），有两个游泳池CHIKV检测为阳性，一个游泳池ZIKV检测为阳性，表明通过卵巢传播产生的后代具有潜在的传染性。
结论：总之，在埃及伊蚊的CHIKV和ZIKV的垂直传播机制的演示是对卫生当局的警报，由于这些疾病仍然被低估，他们的主要城市媒介可能已经获得了这种能力，有助于这些感染的传播。
方法：从戈伊尼亚的不同地区收集埃及伊蚊卵，并在受控的实验室条件下培养，直到成年蚊子出现。成年雌性被分组到包含头部和胸部的池中。随后使用逆转录定量聚合酶链反应(RT-qPCR)测定来评估这些池。
结果：共分析了157个池（N=1570），有两个游泳池CHIKV检测为阳性，一个游泳池ZIKV检测为阳性，表明通过卵巢传播产生的后代具有潜在的传染性。
结论：总之，在埃及伊蚊的CHIKV和ZIKV的垂直传播机制的演示是对卫生当局的警报，由于这些疾病仍然被低估，他们的主要城市媒介可能已经获得了这种能力，有助于这些感染的传播。
