METHODS: Aedes aegypti eggs were collected from different regions of Goiânia and cultivated under controlled laboratory conditions until the emergence of adult mosquitoes. Adult females were grouped into pools containing their heads and thoraxes. These pools were subsequently evaluated using reverse-transcription quantitative polymerase chain reaction (RT-qPCR) assay.
RESULTS: A total of 157 pools (N=1570) were analyzed, with two pools testing positive for CHIKV and one pool testing positive for ZIKV, indicating that the offspring resulting from transovarian transmission are potentially infectious.
CONCLUSIONS: In summary, the demonstration of the vertical transmission mechanisms of CHIKV and ZIKV in A. aegypti serves as an alert to health authorities, as these diseases are still underreported, and their primary urban vector has likely acquired this capacity, contributing to the dissemination of these infections.
方法:从戈伊尼亚的不同地区收集埃及伊蚊卵,并在受控的实验室条件下培养,直到成年蚊子出现。成年雌性被分组到包含头部和胸部的池中。随后使用逆转录定量聚合酶链反应(RT-qPCR)测定来评估这些池。
结果:共分析了157个池(N=1570),有两个游泳池CHIKV检测为阳性,一个游泳池ZIKV检测为阳性,表明通过卵巢传播产生的后代具有潜在的传染性。
结论:总之,在埃及伊蚊的CHIKV和ZIKV的垂直传播机制的演示是对卫生当局的警报,由于这些疾病仍然被低估,他们的主要城市媒介可能已经获得了这种能力,有助于这些感染的传播。