PDF(Pubmed)
Abstract：
Objective : This research investigates the alleles of Variable Number of Tandem Repeats (VNTR) intron 8 of the gene SLC6A3 with attention-deficit / hyperactivity disorder (ADHD) in children and adolescents. Method : The study\'s target population consisted of children and adolescents referred to the specialized clinic, as well as students attending school in Rasht city during 2021-2022. A sample of 95 children between the ages of 6 and 10 with ADHD was selected as the ADHD group, and 95 healthy children were selected as the control group using purposive sampling. The subjects completed the Child Symptom Inventory-4 (CSI-4) checklist after a clinical interview, and demographic information was collected. Genetic sampling was carried out through hair follicles. The sequence of interest was proliferated using the Polymerase Chain Reaction technique )PCR(; afterward, the samples were used for genotype identification on polyacrylamide gel electrophoresis. Results: The chi-square test results indicated that the 5R / 5R genotype (P = 0.026, χ2 = 7.26) and the 5R allele (P = 0.002, χ2 = 9.35) had a higher frequency compared to the control group. Additionally, the odds ratio test indicated that, compared to other genotypes and alleles, the 5R / 5R genotype (OR = 2.75, 95% CI = 1.29-5.82, P = 0.01) and the 5R allele (OR = 2.02, 95% CI = 1.28-3.19, P = 0.002) increase the odds of developing ADHD by 2.7 and 2 times higher, respectively. Conclusion: The present study successfully showed the association between intron 8 gene polymorphism, which is responsible for encoding the dopamine transporter as well as ADHD in children and adolescents in Iran.
摘要：
目的：本研究调查儿童和青少年患有注意缺陷/多动障碍（ADHD）的SLC6A3基因的可变数量串联重复（VNTR）内含子8的等位基因。方法:本研究的目标人群为转诊到专科门诊的儿童和青少年,以及2021-2022年期间在拉什特市上学的学生。选取95名年龄在6至10岁之间的多动症患儿作为多动症组,采用目的性抽样的方法选择95例健康儿童作为对照组。受试者在临床访谈后完成了儿童症状量表-4（CSI-4）清单，并收集了人口统计信息。通过毛囊进行遗传取样。使用聚合酶链反应技术扩增感兴趣的序列)PCR(;之后，样品用于聚丙烯酰胺凝胶电泳的基因型鉴定。结果:卡方检验结果显示,5R/5R基因型(P=0.026,χ2=7.26)和5R等位基因(P=0.002,χ2=9.35)频率高于对照组。此外,赔率比测试表明，与其他基因型和等位基因相比，5R/5R基因型（OR=2.75，95％CI=1.29-5.82，P=0.01）和5R等位基因（OR=2.02，95％CI=1.28-3.19，P=0.002）增加了发生ADHD的几率2.7倍和2倍。分别。结论:本研究成功地显示了内含子8基因多态性,它负责编码多巴胺转运蛋白以及伊朗儿童和青少年的多动症。
