METHODS: Using a viral metagenomics approach, we analyzed the virome of tissues of the dead captive red-crowned crane with diarrhea symptoms in Dongying Biosphere Reserve, Shandong Province, China and feces of individual birds breeding at the corresponding captive breeding center, which were pooled separately.
RESULTS: There is much more DNA and RNA viruses in the feces than that of the tissues. RNA virus belonging to the families Picornaviridae, and DNA viruses belonging to the families Parvoviridae, associated with enteric diseases were detected in the tissues and feces. Genomes of the picornavirus, genomovirus, and parvovirus identified in the study were fully characterized, which further suggested that infectious viruses of these families were possibly presented in the diseased red-crowned crane.
CONCLUSIONS: RNA virus belonging to the families Picornaviridae, and DNA viruses belonging to the families Genomoviridae and Parvoviridae were possibly the causative agent for diarrhea of red-crowned crane. This study has expanded our understanding of the virome of red-crowned crane and provides a baseline for elucidating the etiology for diarrhea of the birds.
方法:使用病毒宏基因组学方法,我们分析了东营生物圈保护区内因腹泻症状死亡的圈养丹顶鹤的组织病毒,山东省,中国和粪便的个体鸟类在相应的圈养繁殖中心繁殖,分别汇集。
结果:粪便中的DNA和RNA病毒比组织中的要多得多。RNA病毒属于小RNA科,和属于细小病毒科的DNA病毒,在组织和粪便中检测到与肠道疾病相关的疾病。小核糖核酸病毒的基因组,基因病毒,研究中鉴定出的细小病毒得到了充分的表征,这进一步表明,这些家族的传染性病毒可能存在于患病的丹顶鹤中。
结论:RNA病毒属于小RNA科,属于基因组病毒科和细小病毒科的DNA病毒可能是丹顶鹤腹泻的病原体。这项研究扩大了我们对丹顶鹤病毒学的理解,并为阐明鸟类腹泻的病因提供了基线。