Abstract：
Prosthetic biomaterials can affect the composition of the subgingival microbiota and consequently the production of proinflammatory cytokines, causing damage to the periodontium. A total of 40 patients were divided into two groups: 20 with monolithic zirconia (MZ) prostheses and 20 with porcelain fused to metal (PFM) with nickel-chromium (Ni-Cr) alloy prostheses. Subgingival plaque and gingival crevicular fluid samples were taken. The Checkerboard technique for DNA-DNA hybridization and the enzyme-linked immunosorbent assay technique were performed. Teeth with MZ presented a lower percentage of bleeding on probing and tooth mobility compared to teeth with PFM with Ni-Cr alloy. Prosthodontic teeth harbored higher total levels of the 18 bacterial species than non-prosthodontic teeth. There was a higher prevalence of S. gordonii and V. parvula species in PFM with Ni-Cr alloy compared to MZ. There was an increase in IL-1β, TNF-α and CX3CL1 levels in PFM with Ni-Cr alloy compared to MZ. MZ is a candidate biomaterial with fewer negative effects on the periodontium, allowing for longer prostheses longevity in the mouth.
摘要：
人工生物材料可以影响龈下微生物群的组成，从而影响促炎细胞因子的产生，对牙周组织造成损害.总共40例患者分为两组：20例采用整体式氧化锆（MZ）假体，20例采用镍铬（Ni-Cr）合金假体与金属熔瓷（PFM）。取龈下菌斑和龈沟液样品。进行了Checkerboard技术的DNA-DNA杂交和酶联免疫吸附测定技术。与使用Ni-Cr合金的PFM的牙齿相比，使用MZ的牙齿在探查和牙齿活动性方面的出血百分比较低。修复牙齿的18种细菌总数高于非修复牙齿。与MZ相比，使用Ni-Cr合金的PFM中的gordonii和细小弧菌的患病率更高。IL-1β增加，与MZ相比，Ni-Cr合金PFM中的TNF-α和CX3CL1水平。MZ是对牙周组织负面影响较小的候选生物材料，允许更长的假肢在口中的寿命。
