Abstract：
Bryophytes, known as poikilohydric plants, possess vegetative desiccation-tolerant (DT) ability to withstand water deficit stress. Consequently, they offer valuable genetic resources for enhancing resistance to water scarcity stress. In this research, we examined the physiological, phytohormonal, and transcriptomic changes in DT mosses Calohypnum plumiforme from two populations, with and without desiccation treatment. Comparative analysis revealed population differentiation at physiological, gene sequence, and expression levels. Under desiccation stress, the activities of superoxide dismutase (SOD) and peroxidase (POD) showed significant increases, along with elevation of soluble sugars and proteins, consistent with the transcriptome changes. Notable activation of the bypass pathway of JA biosynthesis suggested their roles in compensating for JA accumulation. Furthermore, our analysis revealed significant correlations among phytohormones and DEGs in their respective signaling pathway, indicating potential complex interplays of hormones in C plumiforme. Protein phosphatase 2C (PP2C) in the abscisic acid signaling pathway emerged as the pivotal hub in the phytohormone crosstalk regulation network. Overall, this study was one of the first comprehensive transcriptome analyses of moss C. plumiforme under slow desiccation rates, expanding our knowledge of bryophyte transcriptomes and shedding light on the gene regulatory network involved in response to desiccation, as well as the evolutionary processes of local adaptation across moss populations.
摘要：
苔藓植物,被称为多元植物，具有植物耐干燥（DT）能力，可以承受水分亏缺胁迫。因此,它们为增强对缺水压力的抵抗力提供了宝贵的遗传资源。在这项研究中,我们检查了生理，植物激素，和来自两个种群的DT苔藓的转录组变化，有和没有干燥处理。比较分析显示，人口分化在生理上，基因序列,和表达水平。在干燥应力下，超氧化物歧化酶(SOD)和过氧化物酶(POD)活性显著升高,随着可溶性糖和蛋白质的增加，与转录组变化一致。JA生物合成旁路途径的显着激活表明它们在补偿JA积累中的作用。此外，我们的分析揭示了植物激素和DEGs在各自的信号通路中的显著相关性，表明plumi形中激素的潜在复杂相互作用。脱落酸信号通路中的蛋白磷酸酶2C（PP2C）在植物激素串扰调节网络中成为关键枢纽。总的来说,这项研究是在缓慢干燥速率下对苔藓C.plumiforme进行的首批综合转录组分析之一，扩大我们对苔藓植物转录组的认识，并揭示参与干燥反应的基因调控网络，以及苔藓种群局部适应的进化过程。
