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Abstract：
OBJECTIVE: This study aimed to assess the relative gene expression level of transforming growth factor-β1 (TGFB1) and haptoglobin (HP) in the peripheral blood of prostate cancer (PCa) patients and evaluate their diagnostic ability.
METHODS: A total of 125 participants were enrolled in the present study. Among them, 75 PCa patients, 25 benign prostatic hyperplasia (BPH) patients, and 25 healthy volunteers served as the control group. The relative TGFB1 and HP gene expression level was quantified using real-time polymerase chain reaction. Further, free and total PSA levels were determined using electrochemiluminescence assays.
RESULTS: TGFB1 was significantly over-expressed, whereas HP was significantly downregulated in the peripheral blood of PCa patients compared to BPH and control groups (p-value ranges from 0.034 to <0.001). Moreover, the high expression level of TGFB1 was associated with an increased risk of PCa development with OR=1.412 (95%CI: 1.081-1.869, p= 0.012). TGFB1 and HP relative expression levels had lower diagnostic performance to differentiate PCa from normal and BPH individuals compared to PSA, however, the combination of the tested parameters improved the diagnostic efficacy.
CONCLUSIONS: TGFB1 and HP relative expression have moderate diagnostic efficacy in discriminating patients with PCa from BPH and healthy subjects. Furthermore, over-expression of TGFB1 may contribute to the pathogenesis of PCa.
METHODS: A total of 125 participants were enrolled in the present study. Among them, 75 PCa patients, 25 benign prostatic hyperplasia (BPH) patients, and 25 healthy volunteers served as the control group. The relative TGFB1 and HP gene expression level was quantified using real-time polymerase chain reaction. Further, free and total PSA levels were determined using electrochemiluminescence assays.
RESULTS: TGFB1 was significantly over-expressed, whereas HP was significantly downregulated in the peripheral blood of PCa patients compared to BPH and control groups (p-value ranges from 0.034 to <0.001). Moreover, the high expression level of TGFB1 was associated with an increased risk of PCa development with OR=1.412 (95%CI: 1.081-1.869, p= 0.012). TGFB1 and HP relative expression levels had lower diagnostic performance to differentiate PCa from normal and BPH individuals compared to PSA, however, the combination of the tested parameters improved the diagnostic efficacy.
CONCLUSIONS: TGFB1 and HP relative expression have moderate diagnostic efficacy in discriminating patients with PCa from BPH and healthy subjects. Furthermore, over-expression of TGFB1 may contribute to the pathogenesis of PCa.
摘要：
目的：本研究旨在评估前列腺癌（PCa）患者外周血中转化生长因子β1（TGFB1）和结合珠蛋白（HP）的相对基因表达水平，并评估其诊断能力。
方法：本研究共纳入125名参与者。其中,75例PCa患者，25例良性前列腺增生（BPH）患者，25名健康志愿者作为对照组。使用实时聚合酶链反应定量TGFB1和HP基因的相对表达水平。Further,游离PSA水平和总PSA水平采用电化学发光测定法测定.
结果：TGFB1显著过表达，而与BPH组和对照组相比,PCa患者外周血中HP显著下调(p值范围为0.034~<0.001).此外，TGFB1的高表达水平与PCa发展风险增加相关,OR=1.412(95CI:1.081-1.869,p=0.012).与PSA相比，TGFB1和HP相对表达水平在区分PCa与正常个体和BPH个体方面具有较低的诊断性能，然而,测试参数的组合提高了诊断效能.
结论：TGFB1和HP相对表达在鉴别PCa患者与BPH和健康受试者中具有中等诊断功效。此外，TGFB1的过度表达可能与PCa的发病有关。
方法：本研究共纳入125名参与者。其中,75例PCa患者，25例良性前列腺增生（BPH）患者，25名健康志愿者作为对照组。使用实时聚合酶链反应定量TGFB1和HP基因的相对表达水平。Further,游离PSA水平和总PSA水平采用电化学发光测定法测定.
结果：TGFB1显著过表达，而与BPH组和对照组相比,PCa患者外周血中HP显著下调(p值范围为0.034~<0.001).此外，TGFB1的高表达水平与PCa发展风险增加相关,OR=1.412(95CI:1.081-1.869,p=0.012).与PSA相比，TGFB1和HP相对表达水平在区分PCa与正常个体和BPH个体方面具有较低的诊断性能，然而,测试参数的组合提高了诊断效能.
结论：TGFB1和HP相对表达在鉴别PCa患者与BPH和健康受试者中具有中等诊断功效。此外，TGFB1的过度表达可能与PCa的发病有关。
