PDF(Pubmed)
Abstract：
The human milk (HM) microbiota, a highly diverse microbial ecosystem, is thought to contribute to the health benefits associated with breast-feeding, notably through its impact on infant gut microbiota. Our objective was to further explore the role of HM bacteria on gut homeostasis through a \"disassembly/reassembly\" strategy. HM strains covering the diversity of HM cultivable microbiota were first characterized individually and then assembled in synthetic bacterial communities (SynComs) using two human cellular models, peripheral blood mononuclear cells and a quadricellular model mimicking intestinal epithelium. Selected HM bacteria displayed a large range of immunomodulatory properties and had variable effects on epithelial barrier, allowing their classification in functional groups. This multispecies characterization of HM bacteria showed no clear association between taxonomy and HM bacteria impacts on epithelial immune and barrier functions, revealing the entirety and complexity of HM bacteria potential. More importantly, the assembly of HM strains into two SynComs of similar taxonomic composition but with strains exhibiting distinct individual properties, resulted in contrasting impacts on the epithelium. These impacts of SynComs partially diverged from the predicted ones based on individual bacteria. Overall, our results indicate that the functional properties of the HM bacterial community rather than the taxonomic composition itself could play a crucial role in intestinal homeostasis of infants.
摘要：
人乳（HM）微生物群，一个高度多样化的微生物生态系统，被认为有助于与母乳喂养相关的健康益处，特别是通过它对婴儿肠道微生物群的影响。我们的目的是通过“拆解/重组”策略进一步探索HM细菌对肠道稳态的作用。首先对涵盖HM可培养微生物群多样性的HM菌株进行单独表征，然后使用两种人类细胞模型在合成细菌群落（SynComs）中进行组装。外周血单核细胞和模拟肠上皮的四细胞模型。所选择的HM细菌显示出大范围的免疫调节特性，并且对上皮屏障有不同的作用，允许它们在功能组中进行分类。HM细菌的这种多物种特征显示分类学和HM细菌对上皮免疫和屏障功能的影响之间没有明确的关联。揭示了HM细菌潜力的完整性和复杂性。更重要的是,将HM菌株组装成两个分类学组成相似但菌株表现出不同的个体特性的SynComs，对上皮的影响形成对比。SynComs的这些影响与基于单个细菌的预测影响部分不同。总的来说,我们的结果表明，HM细菌群落的功能特性而不是分类学组成本身可能在婴儿的肠道稳态中起关键作用。
