PDF(Pubmed)
Abstract：
Background: Rheumatoid arthritis (RA) is an erosive-destructive inflammation of the joints, and the chronic, long-term stiffness and deformation induced by RA are some of the symptoms of arthritis that are difficult to treat. Dexamethasone (DEX) and melittin (MLT) are two interesting anti-inflammatory substances, both of which possess anti-inflammatory effects exerted through the suppression of the immune system. The purpose of this study was to explore the role of MLT in the treatment of RA by DEX as well as to clarify the influence of MLT on the efficacy and side effects of DEX. Method: The rats were injected with Complete Freund\'s Adjuvant (CFA) to induce arthritis, followed by treatment with different doses of DEX and/or MLT. The relevant indexes of paw inflammation were determined, and the appetite, growth status, arthritis status, cytokine levels, and organ coefficient of the rats were evaluated. In addition, the paraffin sections of the joint tissues were prepared to analyze the pathological changes. Result: DEX exhibited side effects, notably hindering feed intake and growth, and inducing immune organ lesions in the rats. MLT significantly reduced the side effects of DEX and promoted its efficacy. DEX in combination with MLT demonstrated a synergistic efficacy in RA treatment, showing advantages in detumescence reduction, pro-inflammatory cytokine inhibition, and joint internal pathological improvement. Conclusion: Thus, MLT promoted the efficacy of DEX in adjuvant RA treatment in rats, offering an approach to reduce the use dosage and side effects of DEX.
摘要：
背景：类风湿性关节炎（RA）是关节的侵蚀性破坏性炎症，和慢性，RA引起的长期僵硬和变形是关节炎的一些难以治疗的症状。地塞米松（DEX）和蜂毒素（MLT）是两种有趣的抗炎物质，两者都具有通过抑制免疫系统发挥的抗炎作用。本研究的目的是探讨MLT在DEX治疗RA中的作用，并阐明MLT对DEX疗效和副作用的影响。方法:大鼠注射完全弗氏佐剂(CFA)诱导关节炎,然后用不同剂量的DEX和/或MLT治疗。确定爪炎的相关指标,和食欲，生长状态，关节炎状态，细胞因子水平，并对大鼠的脏器系数进行评价。此外,制备关节组织石蜡切片,分析病理改变。结果：DEX出现副作用，特别是阻碍饲料的摄入和生长，诱导大鼠免疫器官损伤。MLT可显著降低DEX的副作用，促进其疗效。DEX联合MLT在RA治疗中表现出协同疗效，在减少消肿方面显示出优势，促炎细胞因子抑制，和关节内部病理改善。结论：因此，MLT促进DEX在大鼠RA辅助治疗中的疗效，提供了一种减少DEX的使用剂量和副作用的方法。
