PDF(Pubmed)
Abstract：
Transurethral catheterization in mice is multifaceted, serving essential functions such as perfusion and drug delivery, and is critical in the development of various urological animal disease models. The complex anatomy of the male mouse urethra presents significant challenges in transurethral catheterization, leading to a predominance of research focused on female specimens. This bias limits the utilization of male mice in lower urinary tract disease studies. Our research aims to develop new reliable methods for transurethral catheterization in adult male mice, thereby expanding their use in relevant disease research. Experiments were conducted on adult male C57BL/6J mice. Utilizing a PE10 catheter measuring 4.5-5 cm in length, the catheter was inserted into the bladder via the mouse\'s urethra under anesthesia. The intubation technique entailed regulating the insertion force, ensuring the catheter\'s lubrication, using a trocar catheter, modifying the catheter\'s trajectory, and accommodating the curvature of the bladder neck. Post-catheter insertion, ultrasound imaging was employed to confirm the catheter\'s accurate positioning within the bladder. Subsequent to catheterization, the bladder was perfused using trypan blue. This method was further validated through its successful application in establishing an acute urinary retention (AUR) model, where the mouse bladder was infused with saline to a pressure of 50 or 80 cm H2O, maintained steadily for 30 min. A thorough morphological assessment of the mouse bladder was conducted after the infusion. Our study successfully pioneered methods for transurethral catheterization in male mice. This technique not only facilitates precise transurethral catheterization but also proves applicable to male mouse models for lower urinary tract diseases, such as AUR.
摘要：
小鼠的经尿道导管插入术是多方面的，提供必要的功能，如灌注和药物输送，并且在各种泌尿系统动物疾病模型的开发中至关重要。雄性小鼠尿道的复杂解剖结构在经尿道导管插入术中提出了重大挑战，导致以女性标本为中心的研究占主导地位。这种偏见限制了雄性小鼠在下尿路疾病研究中的利用。我们的研究旨在开发成年雄性小鼠经尿道插管的新的可靠方法，从而扩大其在相关疾病研究中的用途。在成年雄性C57BL/6J小鼠上进行实验。使用长度为4.5-5厘米的PE10导管，在麻醉下通过小鼠尿道将导管插入膀胱。插管技术需要调节插入力，确保导管的润滑，使用套管针导管，修改导管的轨迹，并适应膀胱颈的曲率。导管插入后,超声成像用于确认导管在膀胱内的准确定位。导管插入术后,用台盼蓝灌注膀胱。该方法在建立急性尿潴留(AUR)模型中的成功应用得到了进一步验证。在小鼠膀胱中注入盐水至50或80厘米H2O的压力，稳定保持30分钟。输注后对小鼠膀胱进行彻底的形态学评估。我们的研究成功地开创了雄性小鼠经尿道插管的方法。该技术不仅有助于精确的经尿道插管，而且证明适用于下尿路疾病的雄性小鼠模型。如Aur。
