PDF(Pubmed)
Abstract：
BACKGROUND: Aphanomyces euteiches is a soil-borne oomycete that causes root rot in pea and other legume species. Symptoms of Aphanomyces root rot (ARR) include root discoloration and wilting, leading to significant yield losses in pea production. Resistance to ARR is known to be polygenic but the roles of single genes in the pea immune response are still poorly understood. This study uses transcriptomics to elucidate the immune response of two pea genotypes varying in their levels of resistance to A. euteiches.
RESULTS: In this study, we inoculated roots of the pea (P. sativum L.) genotypes \'Linnea\' (susceptible) and \'PI180693\' (resistant) with two different A. euteiches strains varying in levels of virulence. The roots were harvested at 6 h post-inoculation (hpi), 20 hpi and 48 hpi, followed by differential gene expression analysis. Our results showed a time- and genotype-dependent immune response towards A. euteiches infection, involving several WRKY and MYB-like transcription factors, along with genes associated with jasmonic acid (JA) and abscisic acid (ABA) signaling. By cross-referencing with genes segregating with partial resistance to ARR, we identified 39 candidate disease resistance genes at the later stage of infection. Among the genes solely upregulated in the resistant genotype \'PI180693\', Psat7g091800.1 was polymorphic between the pea genotypes and encoded a Leucine-rich repeat receptor-like kinase reminiscent of the Arabidopsis thaliana FLAGELLIN-SENSITIVE 2 receptor.
CONCLUSIONS: This study provides new insights into the gene expression dynamics controlling the immune response of resistant and susceptible pea genotypes to A. euteiches infection. We present a set of 39 candidate disease resistance genes for ARR in pea, including the putative immune receptor Psat7g091800.1, for future functional validation.
RESULTS: In this study, we inoculated roots of the pea (P. sativum L.) genotypes \'Linnea\' (susceptible) and \'PI180693\' (resistant) with two different A. euteiches strains varying in levels of virulence. The roots were harvested at 6 h post-inoculation (hpi), 20 hpi and 48 hpi, followed by differential gene expression analysis. Our results showed a time- and genotype-dependent immune response towards A. euteiches infection, involving several WRKY and MYB-like transcription factors, along with genes associated with jasmonic acid (JA) and abscisic acid (ABA) signaling. By cross-referencing with genes segregating with partial resistance to ARR, we identified 39 candidate disease resistance genes at the later stage of infection. Among the genes solely upregulated in the resistant genotype \'PI180693\', Psat7g091800.1 was polymorphic between the pea genotypes and encoded a Leucine-rich repeat receptor-like kinase reminiscent of the Arabidopsis thaliana FLAGELLIN-SENSITIVE 2 receptor.
CONCLUSIONS: This study provides new insights into the gene expression dynamics controlling the immune response of resistant and susceptible pea genotypes to A. euteiches infection. We present a set of 39 candidate disease resistance genes for ARR in pea, including the putative immune receptor Psat7g091800.1, for future functional validation.
摘要：
背景：阿phanomyceseuteiches是一种土壤传播的卵菌，在豌豆和其他豆科植物中引起根腐病。Aphanomyces根腐病（ARR）的症状包括根变色和枯萎，导致豌豆产量显著损失。已知对ARR的抗性是多基因的,但是单个基因在豌豆免疫应答中的作用仍然知之甚少。这项研究使用转录组学来阐明两种豌豆基因型对A.euteiches的抗性水平不同的免疫反应。
结果：在这项研究中，我们接种了豌豆的根(P.sativumL.）基因型\'Linnea\'（易感）和\'PI180693\'（抗性），具有两种不同的A.euteiches菌株，其毒力水平不同。接种后6小时（hpi）收获根，20hpi和48hpi，然后进行差异基因表达分析。我们的结果表明，对A.euteiches感染的时间和基因型依赖性免疫反应，涉及几个WRKY和MYB样转录因子，以及与茉莉酸（JA）和脱落酸（ABA）信号相关的基因。通过与对ARR具有部分抗性的分离基因交叉引用，我们在感染后期鉴定出39个候选抗病基因。在抗性基因型\'PI180693\'中仅上调的基因中，Psat7g091800.1在豌豆基因型之间具有多态性，并编码富含亮氨酸的重复受体样激酶，让人联想到拟南芥FLAGELLIN敏感2受体。
结论：这项研究为控制耐药和易感豌豆基因型对真肠杆菌感染的免疫反应的基因表达动力学提供了新的见解。我们提出了一组39种豌豆ARR的候选抗病基因，包括推定的免疫受体Psat7g091800.1，用于未来的功能验证。
结果：在这项研究中，我们接种了豌豆的根(P.sativumL.）基因型\'Linnea\'（易感）和\'PI180693\'（抗性），具有两种不同的A.euteiches菌株，其毒力水平不同。接种后6小时（hpi）收获根，20hpi和48hpi，然后进行差异基因表达分析。我们的结果表明，对A.euteiches感染的时间和基因型依赖性免疫反应，涉及几个WRKY和MYB样转录因子，以及与茉莉酸（JA）和脱落酸（ABA）信号相关的基因。通过与对ARR具有部分抗性的分离基因交叉引用，我们在感染后期鉴定出39个候选抗病基因。在抗性基因型\'PI180693\'中仅上调的基因中，Psat7g091800.1在豌豆基因型之间具有多态性，并编码富含亮氨酸的重复受体样激酶，让人联想到拟南芥FLAGELLIN敏感2受体。
结论：这项研究为控制耐药和易感豌豆基因型对真肠杆菌感染的免疫反应的基因表达动力学提供了新的见解。我们提出了一组39种豌豆ARR的候选抗病基因，包括推定的免疫受体Psat7g091800.1，用于未来的功能验证。
