Abstract：
BACKGROUND: Recent studies suggest that some nonnutritive sweeteners (NNS) have deleterious effects on the human gut microbiome (HGM). The effect of steviol glycosides on the HGM has not been well studied.
OBJECTIVE: We aimed to evaluate the effects of stevia- compared with sucrose-sweetened beverages on the HGM and fecal short-chain fatty acid (SCFA) profiles.
METHODS: Using a randomized, double-blinded, parallel-design study, n = 59 healthy adults [female/male, n = 36/23, aged 31±9 y, body mass index (BMI): 22.6±1.7 kg/m2] consumed 16 oz of a beverage containing either 25% of the acceptable daily intake (ADI) of stevia or 30 g of sucrose daily for 4 weeks followed by a 4-week washout. At weeks 0 (baseline), 4, and 8, the HGM was characterized via shotgun sequencing, fecal SCFA concentrations were measured using ultra-high performance liquid chromatography-tandem mass spectrometry and anthropometric measurements, fasting serum glucose, insulin and lipids, blood pressure, pulse, and 3-d diet records were obtained.
RESULTS: There were no significant differences in the HGM or fecal SCFA between the stevia and sucrose groups at baseline (P > 0.05). At week 4 (after intervention), there were no significant differences in the HGM at the phylum, family, genus, or species level between the stevia and sucrose groups and no significant differences in fecal SCFA. At week 4, BMI had increased by 0.3 kg/m2 (P = 0.013) in sucrose compared with stevia, but all other anthropometric and cardiometabolic measures and food intake did not differ significantly (P > 0.05). At week 8 (after washout), there were no significant differences in the HGM, fecal SFCA, or any anthropometric or cardiometabolic measure between the stevia and sucrose groups (P > 0.05).
CONCLUSIONS: Daily consumption of a beverage sweetened with 25% of the ADI of stevia for 4 weeks had no significant effects on the HGM, fecal SCFA, or fasting cardiometabolic measures, compared with daily consumption of a beverage sweetened with 30 g of sucrose.
BACKGROUND: clinicaltrials.gov as NCT05264636.
OBJECTIVE: We aimed to evaluate the effects of stevia- compared with sucrose-sweetened beverages on the HGM and fecal short-chain fatty acid (SCFA) profiles.
METHODS: Using a randomized, double-blinded, parallel-design study, n = 59 healthy adults [female/male, n = 36/23, aged 31±9 y, body mass index (BMI): 22.6±1.7 kg/m2] consumed 16 oz of a beverage containing either 25% of the acceptable daily intake (ADI) of stevia or 30 g of sucrose daily for 4 weeks followed by a 4-week washout. At weeks 0 (baseline), 4, and 8, the HGM was characterized via shotgun sequencing, fecal SCFA concentrations were measured using ultra-high performance liquid chromatography-tandem mass spectrometry and anthropometric measurements, fasting serum glucose, insulin and lipids, blood pressure, pulse, and 3-d diet records were obtained.
RESULTS: There were no significant differences in the HGM or fecal SCFA between the stevia and sucrose groups at baseline (P > 0.05). At week 4 (after intervention), there were no significant differences in the HGM at the phylum, family, genus, or species level between the stevia and sucrose groups and no significant differences in fecal SCFA. At week 4, BMI had increased by 0.3 kg/m2 (P = 0.013) in sucrose compared with stevia, but all other anthropometric and cardiometabolic measures and food intake did not differ significantly (P > 0.05). At week 8 (after washout), there were no significant differences in the HGM, fecal SFCA, or any anthropometric or cardiometabolic measure between the stevia and sucrose groups (P > 0.05).
CONCLUSIONS: Daily consumption of a beverage sweetened with 25% of the ADI of stevia for 4 weeks had no significant effects on the HGM, fecal SCFA, or fasting cardiometabolic measures, compared with daily consumption of a beverage sweetened with 30 g of sucrose.
BACKGROUND: clinicaltrials.gov as NCT05264636.
摘要：
背景：最近的研究表明，一些非营养性甜味剂（NNS）对人体肠道微生物组（HGM）具有有害作用。甜菊醇糖苷对HGM的作用尚未得到很好的研究。
目的：我们旨在评估甜菊糖饮料与蔗糖甜味饮料对HGM和粪便短链脂肪酸（SCFA）谱的影响。
方法：使用随机，双盲,并行设计研究，n=59名健康成年人（女/男n=36/23，年龄31±9岁，BMI：22.6±1.7kg/m2）每天消耗16盎司的饮料，其中含有25％的可接受每日摄入量（ADI）的甜叶菊或30克蔗糖，持续4周，然后进行4周的冲洗。在第0周（基线），4和8HGM通过鸟枪测序进行表征，使用超高效液相色谱-串联质谱法和人体测量法测量粪便SCFA浓度，空腹血糖，胰岛素和脂质，血压,获得脉搏和3天饮食记录。
结果：在基线时，甜叶菊和蔗糖组之间的HGM或粪便SCFA没有显着差异（P>0.05）。在第4周（干预后），门的HGM没有显着差异，家庭,甜叶菊和蔗糖组之间的属或种水平，粪便SCFA没有显着差异。在第4周，与甜叶菊相比，蔗糖的BMI增加了0.3kg/m2（P=0.013），但所有其他人体测量和心脏代谢指标以及食物摄入量没有显着差异（P>0.05）。在第8周（冲洗后），HGM没有显着差异，粪便SFCA，或甜叶菊和蔗糖组之间的任何人体测量或心脏代谢测量（P>0.05）。
结论：每日食用甜叶菊25％ADI的饮料4周对HGM没有明显影响，粪便SCFA或空腹心脏代谢测量，与每天食用用30g蔗糖增甜的饮料相比。
背景：Clinicaltrials.gov，如NCT05264636。
目的：我们旨在评估甜菊糖饮料与蔗糖甜味饮料对HGM和粪便短链脂肪酸（SCFA）谱的影响。
方法：使用随机，双盲,并行设计研究，n=59名健康成年人（女/男n=36/23，年龄31±9岁，BMI：22.6±1.7kg/m2）每天消耗16盎司的饮料，其中含有25％的可接受每日摄入量（ADI）的甜叶菊或30克蔗糖，持续4周，然后进行4周的冲洗。在第0周（基线），4和8HGM通过鸟枪测序进行表征，使用超高效液相色谱-串联质谱法和人体测量法测量粪便SCFA浓度，空腹血糖，胰岛素和脂质，血压,获得脉搏和3天饮食记录。
结果：在基线时，甜叶菊和蔗糖组之间的HGM或粪便SCFA没有显着差异（P>0.05）。在第4周（干预后），门的HGM没有显着差异，家庭,甜叶菊和蔗糖组之间的属或种水平，粪便SCFA没有显着差异。在第4周，与甜叶菊相比，蔗糖的BMI增加了0.3kg/m2（P=0.013），但所有其他人体测量和心脏代谢指标以及食物摄入量没有显着差异（P>0.05）。在第8周（冲洗后），HGM没有显着差异，粪便SFCA，或甜叶菊和蔗糖组之间的任何人体测量或心脏代谢测量（P>0.05）。
结论：每日食用甜叶菊25％ADI的饮料4周对HGM没有明显影响，粪便SCFA或空腹心脏代谢测量，与每天食用用30g蔗糖增甜的饮料相比。
背景：Clinicaltrials.gov，如NCT05264636。
