Abstract：
Repetitive transcranial magnetic stimulation (rTMS), a non-invasive brain stimulation technique, holds potential for applications in the treatment of Alzheimer\'s disease (AD). This study aims to compare the therapeutic effects of rTMS at different frequencies on Alzheimer\'s disease and explore the alterations in neuronal electrophysiological properties throughout this process. APP/PS1 AD mice were subjected to two rTMS treatments at 0.5 Hz and 20 Hz, followed by assessments of therapeutic outcomes through the Novel Object Recognition (NOR) and Morris Water Maze (MWM) tests. Following this, whole-cell patch-clamp techniques were used to record action potential, voltage-gated sodium channel currents, and voltage-gated potassium channel currents in dentate gyrus granule neurons. The results show that AD mice exhibit significant cognitive decline compared to normal mice, along with a pronounced reduction in neuronal excitability and ion channel activity. Both frequencies of rTMS treatment partially reversed these changes, demonstrating similar therapeutic efficacy. Furthermore, the investigation indicates that low-frequency magnetic stimulation inhibited the concentrated firing of early action potentials in AD.
摘要：
重复经颅磁刺激(rTMS),一种非侵入性脑刺激技术,在阿尔茨海默病（AD）的治疗中具有应用潜力。本研究旨在比较不同频率的rTMS对阿尔茨海默病的治疗效果，并探讨整个过程中神经元电生理特性的变化。APP/PS1AD小鼠以0.5Hz和20Hz进行两次rTMS处理，然后通过新型物体识别（NOR）和Morris水迷宫（MWM）测试评估治疗结果。在此之后,全细胞膜片钳技术用于记录动作电位，电压门控钠通道电流，齿状回颗粒神经元的电压门控钾通道电流。结果表明，与正常小鼠相比，AD小鼠表现出明显的认知能力下降，随着神经元兴奋性和离子通道活性的显着降低。两种频率的rTMS治疗部分逆转了这些变化，表现出相似的治疗效果。此外，研究表明，低频磁刺激抑制了AD早期动作电位的集中放电。
