Abstract：
IL-8 and IL-10 are crucial inflammatory cytokines that participate in defending host cells against infections. To demonstrate the function of the two interleukin genes in largemouth bass (Micropterus salmoides), we initially cloned and identified the cDNA sequences of il-8 and il-10 in largemouth bass, referred to as Msil-8 and Msil-10, respectively. The open reading frame (ORF) of Msil-8 was 324 bp in length, encoding 107 amino acids, while the ORF of Msil-10 consisted of 726 bp and encoded 241 amino acids. Furthermore, the functional domains of the SCY domain in MsIL-8 and the IL-10 family signature motif in MsIL-10 were highly conserved across vertebrates. Additionally, both MsIL-8 and MsIL-10 showed close relationships with M. dolomieu. Constitutive expression of Msil-8 and Msil-10 was observed in various tissues, with the highest level found in the head kidney. Subsequently, largemouth bass were infected with Nocardia seriolae via intraperitoneal injection to gain a further understanding of the function of these two genes. Bacterial loads were initially detected in the foregut, followed by the midgut, hindgut, and liver. The mRNA expression of Msil-8 was significantly down-regulated after infection, especially at 2 days post-infection (DPI), with a similar expression to Msil-10. In contrast, the expression of Msil-8 and Msil-10 was significantly upregulated in the foregut at 14 DPI. Taken together, these results reveal that the function of IL-8 and IL-10 was likely hindered by N. seriolae, which promoted bacterial proliferation and intercellular diffusion.
摘要：
IL-8和IL-10是重要的炎性细胞因子，参与保护宿主细胞免受感染。为了证明大嘴鲈鱼（Micropterussalmoides）中两个白介素基因的功能，我们最初克隆并鉴定了大口鲈鱼中il-8和il-10的cDNA序列，分别称为Msil-8和Msil-10。Msil-8的开放阅读框（ORF）长度为324bp，编码107个氨基酸，Msil-10的ORF由726bp组成，编码241个氨基酸。此外，MsIL-8中SCY结构域的功能域和MsIL-10中IL-10家族特征基序在脊椎动物中高度保守.此外,MsIL-8和MsIL-10均与多洛米菌密切相关。在各种组织中观察到Msil-8和Msil-10的组成型表达，在头肾中发现的最高水平。随后，通过腹膜内注射大口鲈鱼感染诺卡氏菌，以进一步了解这两个基因的功能。细菌负荷最初是在前肠中检测到的，其次是中肠，后肠,还有肝脏.Msil-8的mRNA表达在感染后显著下调,尤其是在感染后2天（DPI），具有与Msil-10类似的表达式。相比之下,Msil-8和Msil-10的表达在14DPI时在前肠中显著上调。一起来看,这些结果表明，IL-8和IL-10的功能可能受到N.seriolae的阻碍，促进细菌增殖和细胞间扩散。
