Abstract：
Mushroom poisonings caused by Amanita phalloides are the leading cause of mushroom-related deaths worldwide. Alpha-Amanitin (α-AMA), a toxic substance present in these mushrooms, is responsible for the resulting hepatotoxicity and nephrotoxicity. The objective of our study was to determine the distribution of α-AMA in Balb/c mice by labeling with Iodine-131. Mice were injected with a toxic dose (1.4 mg/kg) of α-AMA labeled with Iodine-131. The mice were sacrificed at the 1st, 2nd, 4th, 8th, 24th, and 48th hours under anesthesia. The organs of the mice were removed, and their biodistribution was assessed in all experiments. The percent injected dose per gram (ID/g %) value for kidney, liver, lung, and heart tissues at 1st hour were 1.59 ± 0.07, 1.25 ± 0.33, 3.67 ± 0.80 and 1.07 ± 0.01 respectively. This study provides insights into the potential long-term effects of α-AMA accumulation in specific organs. Additionally, this study has generated essential data that can be used to demonstrate the impact of antidotes on the biological distribution of α-AMA in future toxicity models.
摘要：
由蛋黄粉引起的蘑菇中毒是全球蘑菇相关死亡的主要原因。Alpha-Amanitin(α-AMA),这些蘑菇中存在的有毒物质，导致肝毒性和肾毒性。我们研究的目的是通过用碘-131标记来确定α-AMA在Balb/c小鼠中的分布。给小鼠注射毒性剂量(1.4mg/kg)的用碘-131标记的α-AMA。小鼠在1号处死,2nd,第四，8th,24日,麻醉下第48小时.小鼠的器官被切除，并且在所有实验中都评估了它们的生物分布。每克肾脏的注射剂量百分比（ID/g%）值，肝脏,肺，第1小时的心脏组织分别为1.59±0.07、1.25±0.33、3.67±0.80和1.07±0.01。这项研究提供了对特定器官中α-AMA积累的潜在长期影响的见解。此外,这项研究产生了必要的数据，可用于证明解毒剂对未来毒性模型中α-AMA生物分布的影响。
