Abstract：
Sanguisorba officinalis L. possesses detoxifying, analgesic, and hemostatic properties. After charred processing, S. officinalis exhibits significantly enhanced medicinal effects. Currently, most pharmacokinetic studies focus on the chemical constituents of unprocessed S. officinalis. There is limited research on the comparison of chemical constituents before and after processing. This study established a pharmacokinetic method using ultra-high-performance liquid chromatography-tandem mass spectroscopy (UHPLC-MS/MS) to simultaneously determine the levels of four tannin compounds in rat plasma. In negative ion mode, MS/MS detection was performed using an electrospray ionization source. Chromatographic separation was performed using WATERS ACQUITY HSS T3 column (2.1 × 100 mm, 1.8 µm) with a gradient elution of water and acetonitrile as the mobile phase. The pharmacokinetic results indicate that all four compounds reached peak concentrations within 2 h, demonstrating rapid absorption into the bloodstream within the gastrointestinal tract. Notably, the absorption was generally faster in the charred compound of S. officinalis after processing. These four compounds exhibited slower elimination in rat plasma, while in S. officinalis charcoal, the compounds were eliminated more rapidly. The pharmacokinetic results have revealed the pharmacokinetic characteristics of the four analytes in rat plasma which provides valuable reference information for further investigating the in vivo absorption process of S. officinalis after processing.
摘要：
地黄具有解毒作用，镇痛药,和止血特性。经过烧焦处理，S.officinalis表现出显著增强的药用效果。目前,大多数药代动力学研究集中在未加工的巴草的化学成分。对加工前后化学成分的比较研究有限。本研究建立了一种使用超高效液相色谱-串联质谱（UHPLC-MS/MS）同时测定大鼠血浆中四种单宁化合物水平的药代动力学方法。在负离子模式下，使用电喷雾电离源进行MS/MS检测。色谱分离使用WATERSACQUITYHSST3柱(2.1×100mm,1.8µm），以水和乙腈为流动相的梯度洗脱。药代动力学结果表明，所有四种化合物在2h内达到峰值浓度，在胃肠道内迅速吸收到血液中。值得注意的是,在加工后的烧焦的复方中，吸收通常更快。这四种化合物在大鼠血浆中表现出较慢的消除，而在刺槐木炭中，化合物消除得更快。药动学结果揭示了4种分析物在大鼠血浆中的药代动力学特征,为进一步研究加工后的地黄在体内的吸收过程提供了有价值的参考信息。
