Abstract：
This study aims to explore the relationship between altered vitamin D (VitD3) status and ovarian steroidogenesis in muskrats during the breeding and non-breeding seasons. During the breeding season, the ovaries of muskrats were observably enlarged and increased in weight, accompanied by elevated serum and ovarian VitD3 status. Vitamin D receptor (VDR), VitD3 metabolic molecules (CYP2R1, CYP27B1, and CYP24A1), and steroidogenic enzymes were immunolocalized in the ovarian cells of muskrats. The mRNA levels of VDR, CYP2R1, CYP27B1, and steroidogenic enzymes were considerably higher during the breeding season compared to the non-breeding season. RNA-seq analysis revealed a prominent enrichment of vitamin-related and ovarian steroidogenesis pathways. Furthermore, the addition of 1,25(OH)2D3 to the muskrat granulosa cells in vitro increased VDR and steroidogenic enzymes mRNA levels and enhanced the 17β-estradiol level. Overall, these findings supported that VitD3 promotes the secretion of steroid hormones, thereby affecting seasonal changes in ovarian function in the muskrats.
摘要：
本研究旨在探讨在繁殖和非繁殖季节，麝香大鼠维生素D（VitD3）状态改变与卵巢类固醇生成之间的关系。在繁殖季节，麝鼠的卵巢明显增大，体重增加，伴有血清和卵巢VitD3状态升高。维生素D受体(VDR),VitD3代谢分子（CYP2R1，CYP27B1和CYP24A1），并将类固醇生成酶免疫定位在麝香大鼠的卵巢细胞中。VDR的mRNA水平,与非繁殖季节相比，繁殖季节的CYP2R1，CYP27B1和类固醇生成酶要高得多。RNA-seq分析显示维生素相关和卵巢类固醇生成途径的显著富集。此外，在体外向麝香大鼠颗粒细胞中添加1,25（OH）2D3可增加VDR和类固醇生成酶的mRNA水平，并增强17β-雌二醇的水平。总的来说,这些发现支持VitD3促进类固醇激素的分泌，从而影响麝香大鼠卵巢功能的季节性变化。
