RESULTS: In 2018, the osteoarthritis symptoms of brucellosis in the Brucellosis department of a hospital in Inner Mongolia were investigated. Twenty osteoarthritis-associated B. melitensis strains, isolated from the inpatients in Inner Mongolia during 2013-2017, were subjected to whole genome sequencing. The multilocus sequence type (MLST) and core genome SNP (cgSNP) analysis were conducted to detect molecular epidemiological characteristics. The incidence of brucellosis osteoarthritis symptoms in males (85/120, 70.8%) was significantly higher than that in females (35/120, 29.2%), and the age of patients was concentrated between 41 and 60 years old. In silico analyses indicated ST8 was the prevalent sequence type and the transmission of osteoarthritis-associated B. melitensis among different geographical areas. All strains carry virulence genes, including cgs, lpsA, manCoAg, pgm, pmm, virB4, wbdA and wboA.
CONCLUSIONS: Our study showed the close epidemiologically connection of osteoarthritis-associated B. melitensis strains in northern China. And ST8 was the prevalent sequence type which need our attention.
结果:2018年,对内蒙古某医院布氏杆菌病科的骨关节炎症状进行调查。20个与骨关节炎相关的B.melitensis菌株,从2013-2017年内蒙古住院患者中分离,进行全基因组测序。进行多位点序列类型(MLST)和核心基因组SNP(cgSNP)分析以检测分子流行病学特征。男性(85/120,70.8%)明显高于女性(35/120,29.2%),患者的年龄集中在41至60岁之间。计算机分析表明,ST8是普遍存在的序列类型,并在不同地理区域之间传播与骨关节炎相关的B.melitensis。所有菌株都携带毒力基因,包括cgs,lpsA,manCoAg,PGM,pmm,virB4、wbdA和wboA。
结论:我们的研究表明,中国北方与骨关节炎相关的B.melitensis菌株在流行病学上有密切的联系。ST8是最普遍的序列类型,需要我们注意。