PDF(Pubmed)
Abstract：
Previous studies conducted in our lab revealed microbial assemblages to vary significantly between high (ARS-FY-H) and low fillet yield (ARS-FY-L) genetic lines in adult rainbow trout. We hypothesized that a high ARS-FY-H donor microbiome can accelerate somatic growth in microbiome-depleted rainbow trout larvae of the ARS-FY-L line. Germ-depleted larvae of low ARS-FY-L line trout reared in sterile environments were exposed to high- or low-fillet yield-derived microbiomes starting at first feeding for 27 weeks. Despite weight-normalized diets, somatic mass was significantly increased in larvae receiving high fillet yield microbiome cocktails at 27 weeks post-hatch. RNA-seq from fish tails reveals enrichment in NADH dehydrogenase activity, oxygen carrier, hemoglobin complex, gas transport, and respiratory pathways in high fillet yield recolonized larvae. Transcriptome interrogation suggests a relationship between electron transport chain inputs and body weight assimilation, mediated by the gut microbiome. These findings suggest that microbiome payload originating from high fillet yield adult donors primarily accelerates juvenile somatic mass assimilation through respiratory and mitochondrial input modulation. Further microbiome studies are warranted to assess how increasing beneficial microbial taxa could be a basis for formulating appropriate pre-, pro-, or post-biotics in the form of feed additives and lead to fecal transplantation protocols for accelerated feed conversion and fillet yield in aquaculture.
摘要：
在我们实验室进行的先前研究表明，成年虹鳟鱼的高（ARS-FY-H）和低鱼片产量（ARS-FY-L）遗传系之间的微生物组合差异很大。我们假设高ARS-FY-H供体微生物组可以加速ARS-FY-L系微生物组耗尽的虹鳟鱼幼虫的体细胞生长。在无菌环境中饲养的低ARS-FY-L系鳟鱼的无菌幼虫从首次饲喂开始暴露于高或低鱼片产量衍生的微生物群落27周。尽管体重正常化的饮食，孵化后27周，接受高鱼片产量微生物组混合物的幼虫的体细胞质量显着增加。来自鱼尾的RNA-seq揭示了NADH脱氢酶活性的富集，氧载体,血红蛋白复合物,天然气运输,和高鱼片产量的重新定殖幼虫的呼吸途径。转录组询问表明电子传输链输入与体重同化之间存在关系，由肠道微生物组介导。这些发现表明，源自高鱼片的微生物组有效载荷产生的成年供体主要通过呼吸和线粒体输入调节加速了青少年体细胞物质的同化。进一步的微生物组研究是必要的，以评估如何增加有益的微生物类群可以是制定适当的前，pro-,或饲料添加剂形式的后生物，并导致粪便移植方案，以加速水产养殖中的饲料转化和鱼片产量。
