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Abstract：
The prognostic significance of tumor-infiltrating neutrophils in head and neck squamous cell carcinoma (HNSCC) is poorly understood. It is unclear how the presence of neutrophils affects prognosis due to their polarization into cytotoxic N1 or immunosuppressive N2. Therefore, we determined the number of CD66b+ neutrophil granulocytes separately in the stromal and epithelial compartments in cancer tissues from 397 patients with HNSCC. Tumor samples from six historical patient groups were processed into tissue microarrays and stained immunohistochemically. In total, 21.9% were HPV positive (p16+). Neutrophil counts were much lower in the stromal compartment (372 ± 812) than in the epithelial cancer compartment (1040 ± 1477) (p < 0.001), with large differences between groups. In three groups with high neutrophil infiltration, high rates were associated with a favorable prognosis, whereas in two groups, high rates were a negative prognostic factor. In p16- oropharyngeal and hypopharyngeal cancer high infiltration was associated with a favorable prognosis. Cancers with an exclusion of neutrophils in the epithelial compartment were associated with improved prognosis. In oropharyngeal and hypopharyngeal HPV-negative cancer high neutrophil infiltration rates were clearly associated with prolonged survival. Neutrophil granulocytes in HNSCC may contribute to a favorable or unfavorable prognosis.
摘要：
头颈部鳞状细胞癌（HNSCC）中肿瘤浸润的中性粒细胞的预后意义尚不清楚。目前尚不清楚中性粒细胞的存在如何影响预后，因为它们极化为细胞毒性N1或免疫抑制N2。因此,我们分别测定了397例HNSCC患者癌组织间质和上皮区室中CD66b+中性粒细胞的数量.将来自六个历史患者组的肿瘤样品处理到组织微阵列中并进行免疫组织化学染色。总的来说,21.9%为HPV阳性(p16+)。中性粒细胞计数在基质室（372±812）比在上皮癌室（1040±1477）低得多（p<0.001），组间差异很大。在高中性粒细胞浸润的三组中，高发病率与良好的预后相关，而在两组中，高利率是一个负面的预后因素。在p16-口咽和下咽癌中，高浸润与良好的预后有关。上皮区室中性粒细胞排除的癌症与改善的预后相关。在口咽和下咽HPV阴性癌中，高中性粒细胞浸润率与延长生存期明显相关。HNSCC中的中性粒细胞可能导致有利或不利的预后。
