PDF(Pubmed)
Abstract：
It is unclear whether normal human skin tissue or abnormal scarring are photoreceptive. Therefore, this study investigated photosensitivity in normal skin tissue and hypertrophic scars. The expression of opsins, which are photoreceptor proteins, in normal dermal fibroblasts (NDFs) and hypertrophic scar fibroblasts (HSFs) was examined. After exposure to blue light (BL), changes in the expression levels of αSMA and clock-related genes, specifically PER2 and BMAL1, were examined in both fibroblast types. Opsins were expressed in both fibroblast types, with OPN3 exhibiting the highest expression levels. After peripheral circadian rhythm disruption, BL induced rhythm formation in NDFs. In contrast, although HSFs showed changes in clock-related gene expression levels, no distinct rhythm formation was observed. The expression level of αSMA was significantly higher in HSFs and decreased to the same level as that in NDFs upon BL exposure. When OPN3 knocked-down HSFs were exposed to BL, the reduction in αSMA expression was inhibited. This study showed that BL exposure directly triggers peripheral circadian synchronization in NDFs but not in HSFs. OPN3-mediated BL exposure inhibited HSFs. Although the current results did not elucidate the relationship between peripheral circadian rhythms and hypertrophic scars, they show that BL can be applied for the prevention and treatment of hypertrophic scars and keloids.
摘要：
尚不清楚正常人皮肤组织或异常瘢痕是否具有感光性。因此,这项研究调查了正常皮肤组织和肥厚性瘢痕的光敏性。视蛋白的表达，是感光蛋白，检查了正常真皮成纤维细胞（NDF）和肥厚性瘢痕成纤维细胞（HSF）。暴露于蓝光（BL）后，αSMA和时钟相关基因表达水平的变化，特别是PER2和BMAL1，在两种成纤维细胞类型中进行了检查。视蛋白在两种成纤维细胞中都有表达，OPN3表现出最高的表达水平。外周昼夜节律中断后，BL诱导NDF的节律形成。相比之下,尽管HSF显示时钟相关基因表达水平的变化，没有观察到明显的节律形成。BL暴露后，HSF中αSMA的表达水平显着升高，而与NDF中的表达水平相同。当OPN3敲低的HSF暴露于BL时，αSMA表达的减少受到抑制。这项研究表明，BL暴露直接触发NDF而不是HSF中的外周昼夜节律同步。OPN3介导的BL暴露抑制HSF。虽然目前的结果没有阐明外周昼夜节律和肥厚性瘢痕之间的关系，结果表明，BL可用于肥厚性瘢痕和瘢痕疙瘩的预防和治疗。
