PDF(Pubmed)
Abstract：
The raccoon dog (Nyctereutes procyonoides) is a typical omnivore possessing wide dietary adaptability and tolerance to rough feeding, which may be attributed to its intestinal microbiota. The study aimed to investigate the effect of dietary alfalfa meal levels on the growth performance, nutrient apparent digestibility, serum parameters, and intestinal microbiota of raccoon dogs. Sixty raccoon dogs were randomly divided into four dietary treatments containing 0% (AM0), 5% (AM5), 10% (AM10), and 15% (AM15) alfalfa meal for a 60-day experiment. The results showed that compared to raccoon dogs fed the AM0 diet, those fed the AM5 and AM10 diets had no significant difference in growth performance, while those fed the AM15 diet experienced a significant decrease. Raccoon dogs fed the AM5 diet had no significant effect on the nutrient apparent digestibility. Dietary supplementation with alfalfa meal significantly decreased serum urea levels and increased the antioxidant capacity of raccoon dogs. The intestinal microbiome analysis showed that the richness and diversity of colonic microbiota significantly increased in the AM15 group. With the increase in dietary alfalfa meal levels, the relative abundance of fiber-degrading bacteria in the colon of raccoon dogs, such as Treponema, Phascolarctobacterium, and Christensenellaceae R-7 group, increased. However, the relative abundance of pathogenic bacteria, including Anaerobiospirillum, decreased. In conclusion, the inclusion of 5% alfalfa meal in the raccoon dogs\' diet had no effect on growth performance, but it exhibited the potential to improve serum antioxidant capacity and intestinal microbiota. This indicates that raccoon dogs have a certain tolerance to the addition of alfalfa meal in their diet.
摘要：
浣熊犬（Nyctereutesprocyonoides）是一种典型的杂食动物，具有广泛的饮食适应性和对粗食的耐受性，这可能归因于它的肠道微生物群。本研究旨在探讨饲粮中苜蓿粉水平对苜蓿生长性能的影响,养分表观消化率，血清参数，和浣熊狗的肠道微生物群。将60只浣熊犬随机分为四种含0%(AM0)的饮食处理，5%（AM5），10%(AM10),和15％（AM15）苜蓿粉进行60天的实验。结果表明，与饲喂AM0日粮的浣熊犬相比，饲喂AM5和AM10日粮的生长性能没有显着差异，而那些喂食AM15饮食的人经历了显著的下降。饲喂AM5日粮的浣熊犬对养分表观消化率没有显著影响。饲粮补充苜蓿粉显著降低了浣熊犬的血清尿素水平，提高了其抗氧化能力。肠道微生物组分析表明，AM15组结肠微生物的丰富度和多样性显著增加。随着苜蓿膳食水平的增加，浣熊犬结肠中纤维降解细菌的相对丰度，比如密螺旋体,相颈杆菌,和ChristensenellaceaeR-7组，增加。然而,病原菌的相对丰度，包括厌氧螺旋藻,decreased.总之,在浣熊犬的饮食中含有5%的苜蓿粉对生长性能没有影响，但它显示出改善血清抗氧化能力和肠道菌群的潜力。这表明浣熊犬对饮食中添加苜蓿粉具有一定的耐受性。
