Abstract:
BACKGROUND: Mixed strongylid infections significantly impact equine health and performance. Traditional microscopy-based methods exhibit limitations in accurately identifying strongylid species. Nemabiome deep amplicon sequencing approach previously succeeded in describing the strongylid communities in livestock including equids. However, there are no available studies that describe the structural communities of strongylid parasites in horses in Thailand. Therefore, this study was undertaken encompassing the ITS-2 rDNA metabarcoding assay to characterize strongylid species within horse fecal samples collected from a cohort of yearlings at the largest domesticated stud farm in Thailand. In addition, to investigate the capability of ITS-2 rDNA in assessing the phylogenetic relationships among the identified strongylid species.
RESULTS: The study identified 14 strongylid species in the examined equine populations, each with varying prevalence. Notably, Cylicocyclus nassatus and Cylicostephanus longibursatus were identified as the predominant species, with Strongylus spp. conspicuously absent. The phylogenetic analysis of 207 amplicon sequence variants (ASVs) displayed a complex relationship among the investigated cyathostomin species, with some species are positioned across multiple clades, demonstrating close associations with various species and genera.
CONCLUSIONS: The ITS-2 nemabiome sequencing technique provided a detailed picture of horse strongylid parasite species in the studied population. This establishes a foundation for future investigations into the resistance status of these parasites and enables efforts to mitigate their impact.
摘要:
背景:混合性强直性感染显著影响马的健康和性能。传统的基于显微镜的方法在准确识别strongylid物种方面表现出局限性。Nemabiome深度扩增子测序方法以前成功地描述了包括马匹在内的牲畜中的strongylid群落。然而,没有可用的研究描述了泰国马中strongylid寄生虫的结构群落。因此,这项研究是通过ITS-2rDNAmetabarcoding测定法进行的,以表征从泰国最大的驯化种马农场的一群一岁鸟收集的马粪便样本中的strongylid物种。此外,研究ITS-2rDNA在评估已鉴定的stronylid物种之间的系统发育关系中的能力。
结果:该研究在所检查的马种群中确定了14种强直性物种,每个都有不同的患病率。值得注意的是,cylicocylusnassatus和cylicostephanuslongibursatus被确定为主要物种,与Strongylusspp。明显缺席。207个扩增子序列变体(ASV)的系统发育分析显示了所研究的cyathostomin物种之间的复杂关系,有些物种位于多个分支中,与各种物种和属密切相关。
结论:ITS-2线虫组测序技术提供了所研究种群中马strongylid寄生虫物种的详细图片。这为未来研究这些寄生虫的抗性状况奠定了基础,并能够努力减轻其影响。