Abstract:
Objective: The objective of this study is to evaluate the potential effects of photobiomodulation (PBM) on cell proliferation and extracellular matrix production of human fibroblasts (FN1) cultured in 2D. Background: Patients with healing difficulties suffer injuries that take time to recover. In addition, aging can be seen in our faces daily when we look in the mirror; in both situations, collagen production is reduced. Fibroblasts act in the beginning and at the end of the inflammation phase, signaling to immune agents, and platelets, and producing collagen, coordinating repair. PBM increases cell viability, proliferation, and mRNA production. Methods: Human fibroblasts were irradiated three times after cell seed (after 24, 48, and 72 h) using a gallium-aluminum arsenideGaAlAs low-level laser (LLL). Cell viability, proliferative response, synthesis of collagen types I and III, and soluble collagen production were analyzed. The statistical significance of differences between groups was determined using unpaired one-way analysis of variance (ANOVA) p < 0.05. Results: PBM increased significantly the number of fibroblasts, and the production of collagen types I (Col I) and III (Col III), after three sessions of LLL with 2.5 J per session, every 24 h, for 3 consecutive days; total energy delivered after 72 h is 7.5 J. Conclusions: This energy density of LLL increases fibroblast proliferation and collagen production in vitro without side effects.
摘要:
目的:本研究的目的是评估光生物调节(PBM)对2D培养的人成纤维细胞(FN1)的细胞增殖和细胞外基质产生的潜在影响。背景:愈合困难的患者受伤需要时间才能恢复。此外,每天照镜子时,我们的脸上都可以看到衰老;在这两种情况下,胶原蛋白的产生减少。成纤维细胞在炎症阶段的开始和结束时发挥作用,向免疫剂发出信号,和血小板,产生胶原蛋白,协调修复。PBM增加细胞活力,扩散,和mRNA生产。方法:使用镓铝砷GaAlAs低水平激光(LLL)在细胞种子后(24、48和72h后)照射人成纤维细胞三次。细胞活力,增殖反应,I型和III型胶原蛋白的合成,和可溶性胶原蛋白的产生进行了分析。使用非配对单向方差分析(ANOVA)p<0.05确定组间差异的统计学显著性。结果:PBM显著增加成纤维细胞数量,以及I型胶原蛋白(ColI)和III型胶原蛋白(ColIII)的产生,在三次LLL会议之后,每次会议2.5J,每24小时,连续3天;72小时后递送的总能量为7.5J。结论:LLL的这种能量密度在体外增加成纤维细胞增殖和胶原蛋白产生而无副作用。