PDF(Pubmed)
Abstract：
Apicomplexan Sarcocystis and Trichinella nematodes are food-borne parasites whose life cycle is carried-out in various wildlife and domestic animals. The gray wolf (Canis lupus) is an apex predator acting as an ecosystem engineer. This study aimed to identify the species of Sarcocystis and Trichinella found in the muscles of gray wolves in Lithuania. During the 2017-2022 period, diaphragm, heart, and hind leg samples of 15 animals were examined. Microscopical analysis showed the presence of two types of Sarcocystis parasites in 26.7% of the analyzed muscle samples. Based on the sequencing of five loci, nuclear 18S rDNA, 28S rDNA, ITS1, mitochondrial cox1, and apicoplast rpoB, S. arctica, and S. svanai were identified. The current work presents the first report of S. svanai in gray wolf. Phylogenetically, S. svanai clustered together with S. lutrae, infecting various carnivorans, and S. arctica was most closely related to S. felis from domestic cats. Trichinella spp. were found in 12 gray wolves (80%). For the first time, Trichinella species were molecularly identified in gray wolves from Lithuania. Trichinella britovi was confirmed in all of the isolated Trichinella larvae using a multiplex PCR. Gray wolves in Lithuania may serve as a major source of zoonotic pathogens due to the presence of these parasites.
摘要：
Apicomplex孢子虫和毛毛虫线虫是食源性寄生虫，其生命周期在各种野生动植物和家畜中进行。灰狼（Canis狼疮）是作为生态系统工程师的顶级捕食者。这项研究旨在确定在立陶宛灰狼肌肉中发现的肉孢子虫和旋毛虫的种类。在2017-2022年期间，隔膜,心,并检查了15只动物的后腿样本。显微镜分析表明，在26.7％的分析肌肉样品中存在两种类型的肉囊虫寄生虫。基于五个基因座的测序，核18SrDNA,28SrDNA,ITS1，线粒体cox1和顶端塑料rpoB，S、北极，和S.svanai被确认。目前的工作提出了S.svanai在灰狼中的第一份报告。系统发育,S.svanai和S.lutrae聚集在一起，感染各种食肉动物，和S.arctica是最密切相关的S.felis从家猫。旋毛虫属。在12只灰狼（80％）中发现。第一次,在立陶宛的灰狼中对旋毛虫物种进行了分子鉴定。使用多重PCR在所有分离的旋毛虫幼虫中确认了短枝旋毛虫。由于存在这些寄生虫，立陶宛的灰狼可能是人畜共患病原体的主要来源。
