PDF(Pubmed)
Abstract：
In this study, an actinomycete was isolated from sea mud. The strain K1 was identified as Saccharomonospora sp. by 16S rDNA. The optimal enzyme production temperature, initial pH, time, and concentration of the inducer of this actinomycete strain K1 were 37 °C, pH 8.5, 72 h, and 2% dextran T20 of medium, respectively. Dextranase from strain K1 exhibited maximum activity at 8.5 pH and 50 °C. The molecular weight of the enzyme was <10 kDa. The metal ions Sr2+ and K+ enhanced its activity, whereas Fe3+ and Co2+ had an opposite effect. In addition, high-performance liquid chromatography showed that dextran was mainly hydrolyzed to isomaltoheptose and isomaltopentaose. Also, it could effectively remove biofilms of Streptococcus mutans. Furthermore, it could be used to prepare porous sweet potato starch. This is the first time a dextranase-producing actinomycete strain was screened from marine samples.
摘要：
在这项研究中,从海泥中分离出一种放线菌。菌株K1被鉴定为糖单孢菌。16SrDNA最佳产酶温度,初始pH值,时间,该放线菌K1的诱导剂浓度为37°C，pH8.5，72小时，和2%右旋糖酐T20的培养基，分别。来自菌株K1的葡聚糖酶在8.5pH和50°C时表现出最大活性。该酶的分子量<10kDa。金属离子Sr2+和K+增强了其活性,而Fe3+和Co2+具有相反的作用。此外,高效液相色谱显示葡聚糖主要水解为异麦芽庚糖和异麦芽戊糖。此外，能有效去除变形链球菌的生物膜。此外，可用于制备多孔甘薯淀粉。这是首次从海洋样品中筛选出产葡聚糖酶的放线菌菌株。
