PDF(Pubmed)
Abstract：
Upon exploring the mitotype diversity of the aphid-hunting wasp, Diodontus tristis, we revealed specimens with highly divergent mitotypes from two localities in Lithuania and nesting in clayey substrate, while the specimens with typical mitotypes were found nesting in sandy sites. The comparison of inter- and intra-specific distances and application of delimitation algorithms supported the species status of the clay-nesting populations. Using a set of DNA markers that included complete or partial sequences of six mitochondrial genes, three markers of ribosomal operon, two homeobox genes, and four other nuclear genes, we clarified the phylogenetic relationships of the new cryptic species. The endosymbiotic bacteria infestation was checked, considering the option that the divergent populations may represent clades isolated by Wolbachia infection; however, it did not demonstrate any specificity. We found only subtle morphological differences in the new clay-nesting species, D. argillicola sp. nov.; the discriminant analysis of morphometric measurements did not reliably segregate it as well. Thus, we provide the molecular characters of the cryptic species, which allow confident identification, its phylogenetic position within the genus, and an updated identification key for the D. tristis species group.
摘要：
在探索蚜虫狩猎黄蜂的有丝分裂型多样性后，DiodontusTristis,我们发现了来自立陶宛两个地区并在粘土基质中筑巢的高度分歧型的标本，而具有典型线粒体型的标本被发现在沙质部位嵌套。物种间和物种内距离的比较以及定界算法的应用支持了粘土嵌套种群的物种状态。使用一组包括六个线粒体基因的完整或部分序列的DNA标记，核糖体操纵子的三个标记，两个同源异型盒基因，和其他四个核基因，我们阐明了新隐秘物种的系统发育关系。检查了内共生细菌的侵染，考虑到不同种群可能代表被Wolbachia感染隔离的进化枝的选择；然而，它没有表现出任何特异性。我们在新的粘土嵌套物种中发现了细微的形态差异，D.argillicolasp.11月。形态测量的判别分析也不能可靠地将其分离。因此，我们提供了神秘物种的分子特征，允许自信的识别，它在属中的系统发育位置，以及D.Tristis物种组的最新识别密钥。
