Abstract：
Depression has emerged as the predominant psychiatric affliction affecting individuals. Prior research has substantiated the antidepressant properties exhibited by numerous anesthetics. Sevoflurane, a widely utilized inhalant anesthetic in clinical practice, remains relatively uncharted in terms of its specific antidepressant effects. In this study, we used open field test, forced swimming test and novelty-suppressed feeding test to investigate the anxiety and depression-like behaviors in C57BL/6 mice following the inhalation of sevoflurane. We then used western blotting to scrutinized the expression levels of proteins associated with the brain-derived neurotrophic factor (BDNF)-tryosine receptor kinase B (TrkB) pathway in the hippocampus and prefrontal cortex. To further investigate whether sevoflurane exerts antidepressant-like effects via the BDNF-TrkB pathway, we downregulated TrkB expression by administering siRNA into the lateral ventricle. We found that the inhalation of 2.5 % sevoflurane exerted a significant antidepressant-like effect, accompanied by an elevation in p-TrkB expression levels in the hippocampus and prefrontal cortex. Intriguingly, this antidepressant-like effect was abrogated following the downregulation of TrkB expression through the microinjection of siRNA into the lateral ventricle. In conclusion, this study provides evidence supporting the notion that sevoflurane exerts its antidepressant-like effect via the BDNF-TrkB signaling pathway.
摘要：
抑郁症已成为影响个人的主要精神疾病。先前的研究已经证实了许多麻醉剂表现出的抗抑郁特性。七氟醚,在临床实践中广泛使用的吸入麻醉剂，在其特定的抗抑郁作用方面仍然相对未知。在这项研究中,我们使用了露天测试，强迫游泳试验和新颖性抑制喂养试验，以研究吸入七氟醚后C57BL/6小鼠的焦虑和抑郁样行为。然后，我们使用蛋白质印迹法仔细检查了与海马和前额叶皮层中脑源性神经营养因子（BDNF）-酪氨酸受体激酶B（TrkB）途径相关的蛋白质的表达水平。为了进一步研究七氟醚是否通过BDNF-TrkB通路发挥抗抑郁样作用，我们通过向侧脑室施用siRNA下调TrkB的表达。我们发现吸入2.5%的七氟醚具有明显的抗抑郁作用，伴随着海马和前额叶皮层中p-TrkB表达水平的升高。有趣的是，在通过将siRNA显微注射入侧脑室下调TrkB表达后,这种抗抑郁样效应被消除.总之,本研究提供了支持七氟醚通过BDNF-TrkB信号通路发挥抗抑郁样作用的观点的证据.
