Abstract：
BACKGROUND: Low-dose ionizing radiation-induced protection and damage are of great significance among radiation workers. We aimed to study the role of glutathione S-transferase Pi (GSTP1) in low-dose ionizing radiation damage and clarify the impact of ionizing radiation on the biological activities of cells.
RESULTS: In this study, we collected peripheral blood samples from healthy adults and workers engaged in radiation and radiotherapy and detected the expression of GSTP1 by qPCR. We utilized γ-rays emitted from uranium tailings as a radiation source, with a dose rate of 14 μGy/h. GM12878 cells subjected to this radiation for 7, 14, 21, and 28 days received total doses of 2.4, 4.7, 7.1, and 9.4 mGy, respectively. Subsequent analyses, including flow cytometry, MTS, and other assays, were performed to assess the ionizing radiation\'s effects on cellular biological functions. In peripheral blood samples collected from healthy adults and radiologic technologist working in a hospital, we observed a decreased expression of GSTP1 mRNA in radiation personnel compared to the healthy controls. In cultured GM12878 cells exposed to low-dose ionizing radiation from uranium tailings, we noted significant changes in cell morphology, suppression of proliferation, delay in cell cycle progression, and increased apoptosis. These effects were partially reversed by overexpression of GSTP1. Moreover, low-dose ionizing radiation increased GSTP1 gene methylation and downregulated GSTP1 expression. Furthermore, low-dose ionizing radiation affected the expression of GSTP1-related signaling molecules.
CONCLUSIONS: This study shows that low-dose ionizing radiation damages GM12878 cells and affects their proliferation, cell cycle progression, and apoptosis. In addition, GSTP1 plays a modulating role under low-dose ionizing radiation damage conditions. Low-dose ionizing radiation affects the expression of Nrf2, JNK, and other signaling molecules through GSTP1.
RESULTS: In this study, we collected peripheral blood samples from healthy adults and workers engaged in radiation and radiotherapy and detected the expression of GSTP1 by qPCR. We utilized γ-rays emitted from uranium tailings as a radiation source, with a dose rate of 14 μGy/h. GM12878 cells subjected to this radiation for 7, 14, 21, and 28 days received total doses of 2.4, 4.7, 7.1, and 9.4 mGy, respectively. Subsequent analyses, including flow cytometry, MTS, and other assays, were performed to assess the ionizing radiation\'s effects on cellular biological functions. In peripheral blood samples collected from healthy adults and radiologic technologist working in a hospital, we observed a decreased expression of GSTP1 mRNA in radiation personnel compared to the healthy controls. In cultured GM12878 cells exposed to low-dose ionizing radiation from uranium tailings, we noted significant changes in cell morphology, suppression of proliferation, delay in cell cycle progression, and increased apoptosis. These effects were partially reversed by overexpression of GSTP1. Moreover, low-dose ionizing radiation increased GSTP1 gene methylation and downregulated GSTP1 expression. Furthermore, low-dose ionizing radiation affected the expression of GSTP1-related signaling molecules.
CONCLUSIONS: This study shows that low-dose ionizing radiation damages GM12878 cells and affects their proliferation, cell cycle progression, and apoptosis. In addition, GSTP1 plays a modulating role under low-dose ionizing radiation damage conditions. Low-dose ionizing radiation affects the expression of Nrf2, JNK, and other signaling molecules through GSTP1.
摘要：
背景：低剂量电离辐射引起的防护和损害在放射工作者中具有重要意义。我们旨在研究谷胱甘肽S-转移酶Pi（GSTP1）在低剂量电离辐射损伤中的作用，并阐明电离辐射对细胞生物学活性的影响。
结果：在这项研究中，我们收集健康成人和从事放射和放射治疗的工人的外周血样本，并通过qPCR检测GSTP1的表达。我们利用铀尾矿发射的γ射线作为辐射源，剂量率为14μGy/h。GM12878细胞接受这种辐射7、14、21和28天的总剂量为2.4、4.7、7.1和9.4mGy，分别。随后的分析，包括流式细胞术,MTS,和其他化验，进行评估电离辐射对细胞生物学功能的影响。在从健康成年人和在医院工作的放射技师收集的外周血样本中，与健康对照组相比，我们观察到放射人员中GSTP1mRNA的表达降低。在暴露于铀尾矿低剂量电离辐射的培养GM12878细胞中，我们注意到细胞形态的显著变化，抑制增殖，细胞周期进程的延迟，和增加细胞凋亡。这些作用被GSTP1的过表达部分逆转。此外，低剂量电离辐射增加GSTP1基因甲基化并下调GSTP1表达。此外，低剂量电离辐射影响GSTP1相关信号分子的表达。
结论：本研究表明低剂量电离辐射损伤GM12878细胞并影响其增殖，细胞周期进程，和凋亡。此外,GSTP1在低剂量电离辐射损伤条件下起调节作用。低剂量电离辐射影响Nrf2、JNK、和其他信号分子通过GSTP1。
结果：在这项研究中，我们收集健康成人和从事放射和放射治疗的工人的外周血样本，并通过qPCR检测GSTP1的表达。我们利用铀尾矿发射的γ射线作为辐射源，剂量率为14μGy/h。GM12878细胞接受这种辐射7、14、21和28天的总剂量为2.4、4.7、7.1和9.4mGy，分别。随后的分析，包括流式细胞术,MTS,和其他化验，进行评估电离辐射对细胞生物学功能的影响。在从健康成年人和在医院工作的放射技师收集的外周血样本中，与健康对照组相比，我们观察到放射人员中GSTP1mRNA的表达降低。在暴露于铀尾矿低剂量电离辐射的培养GM12878细胞中，我们注意到细胞形态的显著变化，抑制增殖，细胞周期进程的延迟，和增加细胞凋亡。这些作用被GSTP1的过表达部分逆转。此外，低剂量电离辐射增加GSTP1基因甲基化并下调GSTP1表达。此外，低剂量电离辐射影响GSTP1相关信号分子的表达。
结论：本研究表明低剂量电离辐射损伤GM12878细胞并影响其增殖，细胞周期进程，和凋亡。此外,GSTP1在低剂量电离辐射损伤条件下起调节作用。低剂量电离辐射影响Nrf2、JNK、和其他信号分子通过GSTP1。
