Abstract：
Cu2+ was deemed as toxic and the most common heavy metal pollution in the water and food. Meanwhile, endogenous Cu2+ was deeply involved in plenty of physiological and pathological processes of human. Cu2+ imbalance was related to multiple diseases. Here we developed a Cu2+-responsive NIR probe HX, which not only demonstrated obvious color change when subjected to Cu2+, but also showed linear-dependent NIR fluorescence emission to Cu2+ concentration for Cu2+ detection and quantification both in vitro and in vivo. When HX was applied to imaging Cu2+ in the cell or living animals, intracellular Cu2+ fluctuation and Cu2+ accumulation in the liver could be visualized to indicate the copper level in the cell or organs with low background signals. Meanwhile, by applying HX to monitor Cu2+ uptake in the tumor, copper transporter function could be evaluated to screen the patient who are sensitivity to platinum drug.
摘要：
Cu2被认为是有毒的，是水和食物中最常见的重金属污染。同时,内源性Cu2+参与了人类多种生理和病理过程。Cu2+失衡与多种疾病有关。在这里，我们开发了一种Cu2+响应NIR探针HX，当受到Cu2+时，不仅表现出明显的颜色变化，但也显示出线性依赖的近红外荧光发射Cu2+浓度的Cu2+检测和定量在体外和体内。当HX应用于细胞或活体动物中的Cu2+成像时，可以可视化细胞内Cu2波动和肝脏中的Cu2积累，以指示具有低背景信号的细胞或器官中的铜水平。同时,通过应用HX来监测肿瘤中Cu2+的摄取，可以评估铜转运体功能以筛选对铂类药物敏感的患者。
