PDF(Pubmed)
Abstract：
Bone tissue engineering (BTE) involves the design of three-dimensional (3D) scaffolds that aim to address current challenges of bone defect healing, such as limited donor availability, disease transmission risks, and the necessity for multiple invasive surgeries. Scaffolds can mimic natural bone structure to accelerate the mechanisms involved in the healing process. Herein, a crosslinked combination of biopolymers, including gelatin (GEL), chitosan (CS), and hyaluronic acid (HA), loaded with diatom (Di) and β-sitosterol (BS), is used to produce GCH-Di-S scaffold by freeze-drying method. The GCH scaffold possesses a uniform structure, is biodegradable and biocompatible, and exhibits high porosity and interconnected pores, all required for effective bone repair. The incorporation of Di within the scaffold contributes to the adjustment of porosity and degradation, as well as effectively enhancing the mechanical property and biomineralization. In vivo studies have confirmed the safety of the scaffold and its potential to stimulate the creation of new bone tissue. This is achieved by providing an osteoconductive platform for cell attachment, prompting calcification, and augmenting the proliferation of osteoblasts, which further contributes to angiogenesis and anti-inflammatory effects of BS.
摘要：
骨组织工程（BTE）涉及三维（3D）支架的设计，旨在解决骨缺损愈合的当前挑战，例如有限的捐赠者可用性，疾病传播风险，以及多种侵入性手术的必要性。支架可以模拟自然骨结构以加速参与愈合过程的机制。在这里，生物聚合物的交联组合，包括明胶（GEL），壳聚糖（CS），和透明质酸（HA），负载硅藻（Di）和β-谷甾醇（BS），采用冷冻干燥法制备GCH-Di-S支架。GCH支架具有均匀的结构，是可生物降解和生物相容的，并表现出高孔隙率和相互连接的孔隙，所有这些都需要有效的骨修复。Di在支架内的掺入有助于孔隙度和降解的调节，以及有效增强机械性能和生物矿化。体内研究证实了支架的安全性及其刺激新骨组织产生的潜力。这是通过提供用于细胞附着的骨传导平台来实现的，提示钙化，增加成骨细胞的增殖，这进一步有助于BS的血管生成和抗炎作用。
