PDF(Pubmed)
Abstract：
A probiotic-related benefit for the host is inherently linked to metabolic activity and integration in the gut ecosystem. To facilitate these, probiotics are often combined with specific prebiotics in a synbiotic formulation. Here, we propose an approach for improving probiotic metabolic activity and engraftment. By cultivating the probiotic strain in the presence of a specific prebiotic (preconditioning), the bacterial enzymatic machinery is geared towards prebiotic consumption. Today, it is not known if preconditioning constitutes an advantage for the synbiotic concept. Therefore, we assessed the effects galacto-oligosaccharide (GOS) addition and preconditioning on GOS of Limosilactobacillus reuteri DSM 17938 on ex vivo colonic metabolic profiles, microbial community dynamics, and osteoblastogenesis. We show that adding GOS and preconditioning L. reuteri DSM 17938 act on different scales, yet both increase ex vivo short-chain fatty acid (SCFA) production and engraftment within the microbial community. Furthermore, preconditioned supernatants or SCFA cocktails mirroring these profiles decrease the migration speed of MC3T3-E1 osteoblasts, increase several osteogenic differentiation markers, and stimulate bone mineralization. Thus, our results demonstrate that preconditioning of L. reuteri with GOS may represent an incremental advantage for synbiotics by optimizing metabolite production, microbial engraftment, microbiome profile, and increased osteoblastogenesis.
摘要：
对宿主的益生菌相关益处与肠道生态系统中的代谢活动和整合固有地相关。为了促进这些，益生菌通常与特定的益生元组合在合生元配方中。这里,我们提出了一种改善益生菌代谢活性和植入的方法。通过在特定益生元（预处理）的存在下培养益生菌菌株，细菌酶的机制是面向益生元的消费。今天,不知道预处理是否构成合生元概念的优势。因此,我们评估了低聚半乳糖（GOS）添加和预处理对罗伊利氏杆菌DSM17938离体结肠代谢谱的影响，微生物群落动态，和成骨细胞生成。我们表明，添加GOS和预处理罗伊氏乳杆菌DSM17938在不同的尺度上起作用，然而，两者都增加了离体短链脂肪酸（SCFA）的产生和微生物群落内的植入。此外，预处理的上清液或SCFA鸡尾酒反映这些概况降低MC3T3-E1成骨细胞的迁移速度，增加几种成骨分化标志物，并刺激骨骼矿化。因此，我们的结果表明，用GOS预处理罗伊乳杆菌可能代表合生元的增量优势，通过优化代谢物的产生，微生物植入,微生物组概况，并增加成骨细胞的生成。
