PDF(Pubmed)
Abstract：
BACKGROUND: This study evaluates the effectiveness of a combined regimen involving injectable hydrogels for the treatment of experimental myocardial infarction.
METHODS: Myocardial infarction is an acute illness that negatively affects quality of life and increases mortality rates. Experimental models of myocardial infarction can aid in disease research by allowing for the development of therapies that effectively manage disease progression and promote tissue repair.
METHODS: Experimental animal models of myocardial infarction were established using the ligation method on the anterior descending branch of the left coronary artery (LAD).
METHODS: The efficacy of intracardiac injection of hydrogels, combined with cells, drugs, cytokines, extracellular vesicles, or nucleic acid therapies, was evaluated to assess the functional and morphological improvements in the post-infarction heart achieved through the combined hydrogel regimen.
RESULTS: A literature review was conducted using PubMed, Web of Science, Scopus, and Cochrane databases. A total of 83 papers, including studies on 1332 experimental animals (rats, mice, rabbits, sheep, and pigs), were included in the meta-analysis based on the inclusion and exclusion criteria. The overall effect size observed in the group receiving combined hydrogel therapy, compared to the group receiving hydrogel treatment alone, resulted in an ejection fraction (EF) improvement of 8.87% [95% confidence interval (CI): 7.53, 10.21] and a fractional shortening (FS) improvement of 6.31% [95% CI: 5.94, 6.67] in rat models, while in mice models, the improvements were 16.45% [95% CI: 11.29, 21.61] for EF and 5.68% [95% CI: 5.15, 6.22] for FS. The most significant improvements in EF (rats: MD = 9.63% [95% CI: 4.02, 15.23]; mice: MD = 23.93% [95% CI: 17.52, 30.84]) and FS (rats: MD = 8.55% [95% CI: 2.54, 14.56]; mice: MD = 5.68% [95% CI: 5.15, 6.22]) were observed when extracellular vesicle therapy was used. Although there have been significant results in large animal experiments, the number of studies conducted in this area is limited.
CONCLUSIONS: The present study demonstrates that combining hydrogel with other therapies effectively improves heart function and morphology. Further preclinical research using large animal models is necessary for additional study and validation.
METHODS: Myocardial infarction is an acute illness that negatively affects quality of life and increases mortality rates. Experimental models of myocardial infarction can aid in disease research by allowing for the development of therapies that effectively manage disease progression and promote tissue repair.
METHODS: Experimental animal models of myocardial infarction were established using the ligation method on the anterior descending branch of the left coronary artery (LAD).
METHODS: The efficacy of intracardiac injection of hydrogels, combined with cells, drugs, cytokines, extracellular vesicles, or nucleic acid therapies, was evaluated to assess the functional and morphological improvements in the post-infarction heart achieved through the combined hydrogel regimen.
RESULTS: A literature review was conducted using PubMed, Web of Science, Scopus, and Cochrane databases. A total of 83 papers, including studies on 1332 experimental animals (rats, mice, rabbits, sheep, and pigs), were included in the meta-analysis based on the inclusion and exclusion criteria. The overall effect size observed in the group receiving combined hydrogel therapy, compared to the group receiving hydrogel treatment alone, resulted in an ejection fraction (EF) improvement of 8.87% [95% confidence interval (CI): 7.53, 10.21] and a fractional shortening (FS) improvement of 6.31% [95% CI: 5.94, 6.67] in rat models, while in mice models, the improvements were 16.45% [95% CI: 11.29, 21.61] for EF and 5.68% [95% CI: 5.15, 6.22] for FS. The most significant improvements in EF (rats: MD = 9.63% [95% CI: 4.02, 15.23]; mice: MD = 23.93% [95% CI: 17.52, 30.84]) and FS (rats: MD = 8.55% [95% CI: 2.54, 14.56]; mice: MD = 5.68% [95% CI: 5.15, 6.22]) were observed when extracellular vesicle therapy was used. Although there have been significant results in large animal experiments, the number of studies conducted in this area is limited.
CONCLUSIONS: The present study demonstrates that combining hydrogel with other therapies effectively improves heart function and morphology. Further preclinical research using large animal models is necessary for additional study and validation.
摘要：
背景：这项研究评估了涉及可注射水凝胶的联合方案治疗实验性心肌梗塞的有效性。
方法：心肌梗死是一种对生活质量产生负面影响并增加死亡率的急性疾病。心肌梗死的实验模型可以通过允许开发有效管理疾病进展和促进组织修复的疗法来帮助疾病研究。
方法：采用左冠状动脉前降支(LAD)结扎法建立心肌梗死实验动物模型。
方法：心内注射水凝胶的疗效，结合细胞，毒品,细胞因子,细胞外囊泡，或者核酸疗法,评估通过联合水凝胶方案实现的梗死后心脏的功能和形态学改善。
结果：使用PubMed进行了文献综述，WebofScience,Scopus,和Cochrane数据库。共83篇论文，包括对1332只实验动物(大鼠，老鼠,兔子,绵羊,和猪），根据纳入和排除标准纳入荟萃分析.在接受联合水凝胶治疗的组中观察到的总体效果大小，与单独接受水凝胶治疗的组相比，在大鼠模型中,射血分数(EF)改善8.87%[95%置信区间(CI):7.53,10.21],缩短分数(FS)改善6.31%[95%CI:5.94,6.67],而在小鼠模型中，EF改善为16.45%[95%CI:11.29,21.61],FS改善为5.68%[95%CI:5.15,6.22].当使用细胞外囊泡治疗时,观察到EF(大鼠:MD=9.63%[95%CI:4.02,15.23];小鼠:MD=23.93%[95%CI:17.52,30.84])和FS(大鼠:MD=8.55%[95%CI:2.54,14.56];小鼠:MD=5.68%[95%CI:5.15,6.22])的最显著改善。虽然在大型动物实验中取得了显著成果，在这一领域进行的研究数量有限。
结论：本研究表明，水凝胶与其他疗法的结合可有效改善心脏功能和形态。使用大型动物模型的进一步临床前研究对于额外的研究和验证是必要的。
方法：心肌梗死是一种对生活质量产生负面影响并增加死亡率的急性疾病。心肌梗死的实验模型可以通过允许开发有效管理疾病进展和促进组织修复的疗法来帮助疾病研究。
方法：采用左冠状动脉前降支(LAD)结扎法建立心肌梗死实验动物模型。
方法：心内注射水凝胶的疗效，结合细胞，毒品,细胞因子,细胞外囊泡，或者核酸疗法,评估通过联合水凝胶方案实现的梗死后心脏的功能和形态学改善。
结果：使用PubMed进行了文献综述，WebofScience,Scopus,和Cochrane数据库。共83篇论文，包括对1332只实验动物(大鼠，老鼠,兔子,绵羊,和猪），根据纳入和排除标准纳入荟萃分析.在接受联合水凝胶治疗的组中观察到的总体效果大小，与单独接受水凝胶治疗的组相比，在大鼠模型中,射血分数(EF)改善8.87%[95%置信区间(CI):7.53,10.21],缩短分数(FS)改善6.31%[95%CI:5.94,6.67],而在小鼠模型中，EF改善为16.45%[95%CI:11.29,21.61],FS改善为5.68%[95%CI:5.15,6.22].当使用细胞外囊泡治疗时,观察到EF(大鼠:MD=9.63%[95%CI:4.02,15.23];小鼠:MD=23.93%[95%CI:17.52,30.84])和FS(大鼠:MD=8.55%[95%CI:2.54,14.56];小鼠:MD=5.68%[95%CI:5.15,6.22])的最显著改善。虽然在大型动物实验中取得了显著成果，在这一领域进行的研究数量有限。
结论：本研究表明，水凝胶与其他疗法的结合可有效改善心脏功能和形态。使用大型动物模型的进一步临床前研究对于额外的研究和验证是必要的。
