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Abstract：
The aim of our study was to assess if the addition of PRGF to healthy human sperm affects its motility and vitality.
This was a prospective study, with 44 sperm donors on whom sperm analysis was performed. Nine mL of blood was collected and PRGF was obtained using PRGF-Endoret® technology. The influence of different dilutions of PRGF (5%, 10%, 20%, 40%) applied to 15 sperm donors was compared, and sperm motility was assessed after 30 minutes. In the second part of the study, 29 sperm donors were studied to analyze the influence of 20% dilution of PRGF at 15, 30 and 45 minutes in fresh and thawed sperm samples. Motility was assessed after the addition of PRGF and after analysis each aliquot was frozen. After thawing, concentration and motility were assessed at the same time periods.
There were no differences in sperm motility in fresh samples between dilutions of PRGF when assessed 30 minutes after administration, nor between them, nor when compared to the control group immediately prior to treatment. No trend was observed between motility and PRGF dilution in linear regression analysis. There were no significant differences in thawed samples.
The administration of 20% PRGF dilution had no effect on sperm motility compared to samples without PRGF. In addition, there was no change in sperm vitality when comparing samples with and without PRGF. More studies focusing on subnormal sperm samples, analyzing different PRGF concentrations and increasing the number of study variables are needed.
This was a prospective study, with 44 sperm donors on whom sperm analysis was performed. Nine mL of blood was collected and PRGF was obtained using PRGF-Endoret® technology. The influence of different dilutions of PRGF (5%, 10%, 20%, 40%) applied to 15 sperm donors was compared, and sperm motility was assessed after 30 minutes. In the second part of the study, 29 sperm donors were studied to analyze the influence of 20% dilution of PRGF at 15, 30 and 45 minutes in fresh and thawed sperm samples. Motility was assessed after the addition of PRGF and after analysis each aliquot was frozen. After thawing, concentration and motility were assessed at the same time periods.
There were no differences in sperm motility in fresh samples between dilutions of PRGF when assessed 30 minutes after administration, nor between them, nor when compared to the control group immediately prior to treatment. No trend was observed between motility and PRGF dilution in linear regression analysis. There were no significant differences in thawed samples.
The administration of 20% PRGF dilution had no effect on sperm motility compared to samples without PRGF. In addition, there was no change in sperm vitality when comparing samples with and without PRGF. More studies focusing on subnormal sperm samples, analyzing different PRGF concentrations and increasing the number of study variables are needed.
摘要：
目的：我们研究的目的是评估向健康人精子中添加PRGF是否会影响其运动和活力。
方法：这是一项前瞻性研究，对44名精子捐献者进行了精子分析。收集9mL血液并使用PRGF-Endoret®技术获得PRGF。不同稀释度的PRGF(5%，10%,20%,40%)应用于15个精子捐献者进行比较，30分钟后评估精子活力。在研究的第二部分,研究了29个精子供体以分析在新鲜和解冻的精子样品中在15、30和45分钟时PRGF的20%稀释的影响。在添加PRGF之后评估运动性,并且在分析之后将每个等分试样冷冻。解冻后,在相同的时间段评估浓度和运动性。
结果：在给药后30分钟评估时，不同稀释度的PRGF新鲜样品的精子活力没有差异，也不是他们之间，与治疗前的对照组相比，也没有。在线性回归分析中没有观察到运动性和PRGF稀释之间的趋势。在解冻的样品中没有显著差异。
结论:与不含PRGF的样品相比,施用20%PRGF稀释液对精子运动没有影响。此外,比较有和没有PRGF的样本时，精子活力没有变化。更多的研究集中在低于正常的精子样本上，需要分析不同的PRGF浓度并增加研究变量的数量.
方法：这是一项前瞻性研究，对44名精子捐献者进行了精子分析。收集9mL血液并使用PRGF-Endoret®技术获得PRGF。不同稀释度的PRGF(5%，10%,20%,40%)应用于15个精子捐献者进行比较，30分钟后评估精子活力。在研究的第二部分,研究了29个精子供体以分析在新鲜和解冻的精子样品中在15、30和45分钟时PRGF的20%稀释的影响。在添加PRGF之后评估运动性,并且在分析之后将每个等分试样冷冻。解冻后,在相同的时间段评估浓度和运动性。
结果：在给药后30分钟评估时，不同稀释度的PRGF新鲜样品的精子活力没有差异，也不是他们之间，与治疗前的对照组相比，也没有。在线性回归分析中没有观察到运动性和PRGF稀释之间的趋势。在解冻的样品中没有显著差异。
结论:与不含PRGF的样品相比,施用20%PRGF稀释液对精子运动没有影响。此外,比较有和没有PRGF的样本时，精子活力没有变化。更多的研究集中在低于正常的精子样本上，需要分析不同的PRGF浓度并增加研究变量的数量.
