Abstract：
The epidemiological picture of Taenia saginata infections in Kenya is fragmented with limited available data. Although Sarcocystis species are significant meat-borne parasites, few studies have explored their occurrence in Kenya. This study aimed to estimate the occurrence of bovine cysticercosis and screen for the presence of Sarcocystis spp. A meat inspection-based survey was conducted in ten abattoirs in Narok County, Kenya, and inspection for T. saginata cysticerci was limited to the Triceps brachii muscle. The apparent occurrence of the parasite was 5.4% (95% CI, 3.8, 7.6, n=573). Molecular confirmation of T. saginata was done via nested polymerase chain reaction targeting the mitochondrial 12S ribosomal RNA gene and restricted fragment length polymorphism. Sarcocystis species were identified using a multiplex polymerase chain reaction method targeting the 18S ribosomal RNA gene sequences and the mitochondrial cytochrome c oxidase subunit I gene. Of the 31 cystic lesions tested, 26/31 (83.9%) were confirmed to be T. saginata.Sarcocystis cruzi and S. hominis were detected in 8/31 (25.8%) and 1/31 (3.2%) of the cystic lesions, respectively. Co-infections of S. cruzi and T. saginata were found in 6/31 lesions (19.4%). The confirmation of bovine cysticercosis and S. hominis is suggestive of the presence of risky culinary and sanitation practices that facilitate transmission. This is the first report and molecular confirmation of Sarcocystis spp. in cattle in the country. The presence of both zoonotic S. hominis and pathogenic S. cruzi highlights an underexplored concern of veterinary and human health significance, warranting further epidemiological investigation.
摘要：
肯尼亚牛带虫感染的流行病学情况支离破碎，现有数据有限。尽管结节虫是重要的肉类寄生虫，很少有研究探索它们在肯尼亚的发生。本研究旨在评估牛囊虫病的发生情况，并筛选肉囊虫的存在。在纳罗克县的十个屠宰场进行了基于肉类检查的调查，肯尼亚,对囊尾蚴的检查仅限于肱三头肌。寄生虫的明显发生率为5.4％（95％CI，3.8，7.6，n=573）。通过以线粒体12S核糖体RNA基因和限制性片段长度多态性为目标的巢式聚合酶链反应来进行T.saginata的分子确认。使用针对18S核糖体RNA基因序列和线粒体细胞色素c氧化酶亚基I基因的多重聚合酶链反应方法鉴定了结节虫物种。在测试的31个囊性病变中，26/31（83.9%）被证实为萨吉纳塔。在8/31(25.8%)和1/31(3.2%)的囊性病变中检测到克鲁氏结节虫和人源链球菌，分别。在6/31的病变中发现了S.cruzi和T.saginata的共感染（19.4％）。牛囊虫病和人源链球菌的确认表明存在促进传播的危险的烹饪和卫生习惯。这是首次报道和分子确认的肉囊虫。在这个国家的牛。人畜共患S.hominis和致病性S.cruzi的存在凸显了对兽医和人类健康意义的未充分关注，需要进一步的流行病学调查。
