Abstract：
The comparative metabolic profiling and their biological properties of eight extracts obtained from diverse parts (leaves, flowers, roots) of the medicinal plant Flourensia fiebrigii S.F. Blake, a chemotype growing in highland areas (2750 m a.s.l.) of northwest Argentina, were investigated. The extracts were analysed by GC-MS and UHPLC-MS/MS. GC-MS analysis revealed the presence of encecalin (relative content: 24.86 %) in ethereal flower extract (EF) and this benzopyran (5.93 %) together sitosterol (11.35 %) in the bioactive ethereal leaf exudate (ELE). By UHPLC-MS/MS the main compounds identified in both samples were: limocitrin, (22.31 %), (2Z)-4,6-dihydroxy-2-[(4-hydroxy-3,5-dimethoxyphenyl)methylidene]-1-benzofuran-3-one (21.31 %), isobavachin (14.47 %), naringenin (13.50 %), and sternbin, (12.49 %). Phytocomplexes derived from aerial parts exhibited significant activity against biofilm production of Pseudomonas aeruginosa and Staphylococcus aureus, reaching inhibitions of 74.7-99.9 % with ELE (50 μg/mL). Notably, the extracts did not affect nutraceutical and environmental bacteria, suggesting a selective activity. ELE also showed the highest reactive species scavenging ability. This study provides valuable insights into the potential applications of this chemotype.
摘要：
从不同部位（叶，鲜花,根）药用植物FlourensiafiebrigiiS.F.Blake，一种生长在阿根廷西北部高地地区（2750ma.s.l.）的化学型，被调查了。通过GC-MS和UHPLC-MS/MS分析提取物。GC-MS分析显示，在以太花提取物（EF）中存在encecalin（相对含量：24.86％），并且在生物活性以太叶渗出物（ELE）中存在这种苯并吡喃（5.93％）和谷甾醇（11.35％）。通过UHPLC-MS/MS鉴定的两种样品中的主要化合物是：limocitrin，（22.31%），(2Z)-4,6-二羟基-2-[(4-羟基-3,5-二甲氧基苯基)亚甲基]-1-苯并呋喃-3-酮(21.31%),异叶华夏素(14.47%)，柚皮苷,（13.50%），和Sternbin,（12.49%）。来自地上部分的植物复合物对铜绿假单胞菌和金黄色葡萄球菌的生物膜产生具有显着的活性，用ELE（50µg/mL）达到74.7-99.9％的抑制作用。值得注意的是,提取物不影响营养食品和环境细菌，表明有选择性的活动。ELE还显示出最高的反应性物种清除能力。这项研究为这种化学型的潜在应用提供了有价值的见解。
