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Abstract：
Applying extracts from plants is considered a safe approach in biomedicine and bio-nanotechnology. The present report is considered the first study that evaluated the seeds of Lasiurus scindicus and Panicum turgidum as biogenic agents in the synthesis of silver nanoparticles (AgNPs) which had bioactivity against cancer cells and bacteria. Assessment of NPs activity against varied cell lines (colorectal cancer HCT116 and breast cancer MDA MBA 231 and MCF 10A used as control) was performed beside the antibacterial efficiency. Different techniques (DLS, TEM, EDX and FTIR) were applied to characterize the biosynthesized AgNPs. The phytochemicals from both L. scindicus and Panicum turgidum were identified by GC-MS analysis. Spherical monodisperse NPs at average diameters of 149.6 and 100.4 nm were obtained from seed extract of L. scindicus (L-AgNPs) and P. turgidum, (P-AgNPs) respectively. A strong absorption peak at 3 keV is observed by the EDX spectrum in the tested NPs. Our study provided effective NPs in mitigating the tested cell lines and the lowest IC50 were 7.8 and 10.30 for MDA MB231 treated by L-AgNPs and P-AgNPs, respectively. Both fabricated NPs might differentially target the MDA MB231 cells compared to HCT116 and MCF10A. Ultrastructural changes and damage for the NPs-treated MDA MB231 cells were studied using TEM and LSM analysis. Antibacterial activity was also observed. About 200 compounds were identified in L. scindicus and P. turgidum by GC-MS analysis might be responsible for the NPs reduction and capping abilities. Efficient NPs against cancer cells and microbes were obtained, however large-scale screening is needed to validate our findings.
摘要：
应用植物提取物被认为是生物医学和生物纳米技术的安全方法。本报告被认为是第一项研究，该研究评估了Lasiurusscindicus和Panicumturgidum的种子作为合成具有针对癌细胞和细菌的生物活性的银纳米颗粒（AgNPs）的生物因子。除抗菌效率外，还评估了针对不同细胞系（结直肠癌HCT116和乳腺癌MDAMBA231和MCF10A用作对照）的NP活性。不同的技术(DLS，TEM,EDX和FTIR）用于表征生物合成的AgNP。通过GC-MS分析鉴定了苦参和苦参的植物化学物质。平均直径为149.6和100.4nm的球形单分散NP是从苦叶乳杆菌（L-AgNPs）和杜氏疟原虫的种子提取物中获得的，(P-AgNPs)。通过EDX谱在测试的NP中观察到3keV处的强吸收峰。我们的研究提供了有效的NPs来减轻所测试的细胞系，对于L-AgNPs和P-AgNPs处理的MDAMB231，最低的IC50分别为7.8和10.30。分别。与HCT116和MCF10A相比，两种制造的NP都可能不同地靶向MDAMB231细胞。使用TEM和LSM分析研究了NPs处理的MDAMB231细胞的超微结构变化和损伤。还观察到抗菌活性。通过GC-MS分析，在sraindicus和turgidum中鉴定出大约200种化合物可能是NPs减少和封端能力的原因。获得了针对癌细胞和微生物的有效NP，然而,需要大规模筛查来验证我们的发现.
