Abstract：
Programmed cell death-ligand 1 (PDL1) is a transmembrane protein that is characterized as an immune regulatory molecule. We recently developed a recombinant single-chain fragment of variable domain (scFv) against PDL1, which showed high binding efficiency to purified recombinant PDL1 protein. However, at that time, proof-of-concept data for the effect of scFv using PDL1-expressing cells was lacking. In this study, we conducted two kinds of cell-based immunoassays, western blotting and enzyme-linked immunosorbent assay, using anti-PDL1 scFv. The results indicate that scFv can selectively and sensitively detect PDL1 from PDL1 positive human cancer cell lines. Our findings suggest that scFv could be used as a potential PDL1 inhibitor agent and probe for cell-based immunoassays to detect PDL1.
摘要：
程序性细胞死亡配体1（PDL1）是一种跨膜蛋白，其特征是免疫调节分子。我们最近开发了针对PDL1的可变结构域（scFv）的重组单链片段，该片段显示出与纯化的重组PDL1蛋白的高结合效率。然而,当时,缺乏使用PDL1表达细胞的scFv效应的概念验证数据。在这项研究中,我们进行了两种基于细胞的免疫测定，蛋白质印迹和酶联免疫吸附测定，使用抗PDL1scFv。结果表明，scFv可以选择性和灵敏地检测PDL1阳性人类癌细胞系的PDL1。我们的发现表明，scFv可以用作潜在的PDL1抑制剂和基于细胞的免疫测定探针来检测PDL1。
