PDF(Pubmed)
Abstract：
SRPK1 is a splicing-related protein that plays an important role in the development and function of the human brain. This article presents evidence that SRPK1 has distinct spatiotemporal expression patterns enriched in processes related to neurodevelopmental disorders across development.
We used the BrainSpan growing mammalian brain transcriptome to evaluate the distribution of SRPK1 throughout the entire brain. RNA-sequencing data were gathered from 524 tissue samples recovered from 41 postmortem brains of physiologically normal individuals spanning early developing fetus (8 postconception weeks, PCW) to later life (40 years of age). Using the Allen Human Brain Atlas (AHBA) dataset, we analyzed the spatial gene expression of 15 adult human brains. Using Toppgene, we identified genes that exhibit significant coexpression with SRPK1.
We found evidence that analyzing the spatiotemporal gene expression profile and identifying coexpressed genes reveals that SRPK1 expression is involved in various neurodevelopmental and somatic events throughout the lifetime.
Our findings highlight the importance of detailed maps of gene expression in the human brain for improved human-to-human translation and illustrate differences in SRPK1 expression across anatomical areas and developmental stages in healthy human brain tissue.
We used the BrainSpan growing mammalian brain transcriptome to evaluate the distribution of SRPK1 throughout the entire brain. RNA-sequencing data were gathered from 524 tissue samples recovered from 41 postmortem brains of physiologically normal individuals spanning early developing fetus (8 postconception weeks, PCW) to later life (40 years of age). Using the Allen Human Brain Atlas (AHBA) dataset, we analyzed the spatial gene expression of 15 adult human brains. Using Toppgene, we identified genes that exhibit significant coexpression with SRPK1.
We found evidence that analyzing the spatiotemporal gene expression profile and identifying coexpressed genes reveals that SRPK1 expression is involved in various neurodevelopmental and somatic events throughout the lifetime.
Our findings highlight the importance of detailed maps of gene expression in the human brain for improved human-to-human translation and illustrate differences in SRPK1 expression across anatomical areas and developmental stages in healthy human brain tissue.
摘要：
背景：SRPK1是一种剪接相关蛋白，在人脑的发育和功能中起重要作用。本文提供的证据表明，SRPK1在与神经发育障碍相关的过程中具有不同的时空表达模式。
方法：我们使用BrainSpan生长的哺乳动物大脑转录组来评估SRPK1在整个大脑中的分布。RNA测序数据是从从41个生理正常个体的死后大脑中收集的524个组织样本中收集的，这些样本跨越了早期发育中的胎儿（8个受孕后周，PCW）到以后的生活（40岁）。使用艾伦人脑图集（AHBA）数据集，我们分析了15个成年人大脑的空间基因表达。使用Toppgene,我们鉴定了与SRPK1显著共表达的基因.
结果：我们发现，分析时空基因表达谱和鉴定共表达基因的证据表明，SRPK1表达在整个生命周期中参与各种神经发育和体细胞事件。
结论：我们的发现强调了人脑中基因表达的详细图谱对于改善人与人之间的翻译的重要性，并说明了健康人类脑组织中SRPK1表达在解剖区域和发育阶段的差异。
方法：我们使用BrainSpan生长的哺乳动物大脑转录组来评估SRPK1在整个大脑中的分布。RNA测序数据是从从41个生理正常个体的死后大脑中收集的524个组织样本中收集的，这些样本跨越了早期发育中的胎儿（8个受孕后周，PCW）到以后的生活（40岁）。使用艾伦人脑图集（AHBA）数据集，我们分析了15个成年人大脑的空间基因表达。使用Toppgene,我们鉴定了与SRPK1显著共表达的基因.
结果：我们发现，分析时空基因表达谱和鉴定共表达基因的证据表明，SRPK1表达在整个生命周期中参与各种神经发育和体细胞事件。
结论：我们的发现强调了人脑中基因表达的详细图谱对于改善人与人之间的翻译的重要性，并说明了健康人类脑组织中SRPK1表达在解剖区域和发育阶段的差异。
