PDF(Pubmed)
Abstract：
Phenols such as bisphenols, parabens, and triclosan are common environmental endocrine disruptors. Previous epidemiological studies have suggested that phenols may affect semen quality, but the results were inconsistent. In addition, most existing studies have been limited to the effects of a single chemical compound, ignoring the health effects of mixed exposure to multiple chemicals. Thus, we aimed to explore the associations between individual and mixed exposure to phenols and various semen quality parameters. In this study, a rapid and sensitive method was used to determine 18 phenolic compounds in urine samples of 799 volunteers who donated sperm samples to the Shanghai Human Sperm Bank. A spot urine sample was collected from each subject on the day of their clinic visit and stored at -20 ℃ until testing. Urine samples (200 μL) were extracted and added with 20 μL of an internal standard and 50 μL of β-glucuronidase solution. The mixtures were then incubated for 12 h at 37 ℃. After hydrolysis, the samples were extracted twice using ethyl acetate (500 μL). The concentrations of the 18 phenolic compounds were measured using high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Semen quality parameters were analyzed using a computer-aided semen analyzer. Multiple linear regressions were used to detect the associations between individual phenol exposure and semen quality parameters. In addition, weighted quantile sum (WQS) models were used to explore the associations between mixed-phenol exposure and semen quality parameters. After adjusting for potential covariates, the results of multiple linear regressions showed that exposure to ethyl paraben (EtP) was significantly negatively associated with sperm concentration and total sperm count (P<0.05). In addition, exposure to mixed phenols was significantly associated with decreased sperm concentration; methyl paraben (MeP) and EtP were identified as the main contributors to this decrease. Thus, phenol exposure may be associated with decreased semen quality in young males, particularly with respect to sperm concentration and total sperm count.
摘要：
酚类，如双酚类，对羟基苯甲酸酯,和三氯生是常见的环境内分泌干扰物。以前的流行病学研究表明，酚类可能会影响精液质量，但是结果不一致。此外,大多数现有的研究仅限于单一化合物的作用，忽略混合接触多种化学物质对健康的影响。因此，我们的目的是探讨单独和混合接触酚类物质与各种精液质量参数之间的关系。在这项研究中,采用一种快速、灵敏的方法测定了799名志愿者的尿液样本中的18种酚类化合物，这些志愿者将精子样本捐献给了上海人类精子库。在他们的临床访问当天从每个受试者收集斑点尿样,并在-20°C下储存直至测试。提取尿液样品(200μL)并加入20μL内标和50μLβ-葡糖醛酸糖苷酶溶液。然后将混合物在37°C下孵育12小时。水解后，使用乙酸乙酯(500μL)提取样品两次。使用高效液相色谱-串联质谱法(HPLC-MS/MS)测量18种酚类化合物的浓度。使用计算机辅助精液分析仪分析精液质量参数。多元线性回归用于检测个体苯酚暴露与精液质量参数之间的关联。此外,加权分位数和(WQS)模型用于探索混合酚暴露与精液质量参数之间的关联.在调整潜在协变量后，多元线性回归结果显示，对羟基苯甲酸乙酯（EtP）暴露与精子浓度和精子总数呈显著负相关（P<0.05）。此外,暴露于混合酚与精子浓度降低显着相关;对羟基苯甲酸甲酯（MeP）和EtP被确定为这种减少的主要原因。因此，苯酚暴露可能与年轻男性精液质量下降有关，特别是关于精子浓度和精子总数。
